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PXD048587

PXD048587 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleEFHD2 Suppresses Intestinal Inflammation by Blocking Intestinal Epithelial Cell TNFR1 Internalization and Cell Death
DescriptionDeath of intestinal epithelial cells (IECs) is important in the pathological process of intestinal inflammatory diseases. TNF acts as one pathogenic driver for inducing IEC death and substantial intestinal inflammation. However, the physiological protective mechanisms for suppressing TNF-induced IEC death remain poorly understood. Here, we report that EF-hand domain-containing protein D2 (EFHD2), highly expressed in normal intestine tissues but decreased in intestinal biopsy samples of ulcerative colitis patients, protects the intestinal epithelium from TNF-induced IEC apoptosis. EFHD2 inhibits TNF-induced apoptosis in primary IECs and intestinal organoids (enteroids). Mice deficient of Efhd2 in IECs exhibit excessive IEC death and exacerbated experimental colitis. Mechanistically, EFHD2 interacts with Cofilin to suppress the phosphorylation of Cofilin, leading to the inhibition of TNF receptor Ⅰ (TNFR1) internalization and TNF-induced apoptosis. Our findings define EFHD2 as an endogenous suppressor of IEC death to protect intestine inflammation, providing new insight to the regulation of death receptor signaling and control of intestinal inflammatory diseases.
HostingRepositoryiProX
AnnounceDate2024-01-14
AnnouncementXMLSubmission_2024-01-15_21:07:25.577.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterJiacheng Wu
SpeciesList scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-01-15 21:07:14ID requested
12024-01-15 21:07:25announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Intestinal inflammation, EF-hand domain-containing protein D2, Inflammatory bowel disease, Epithelial cell death, TNFR1 internalization
Contact List
Xuetao Cao
contact affiliationDepartment of Immunology, Institute of Basic Medical Sciences, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing
contact emailcaoxt@immunol.org
lab head
Jiacheng Wu
contact affiliationPeking Union Medical College, Chinese Academy of Medical Sciences
contact emailjiachengwu_immunol@outlook.com
dataset submitter
Full Dataset Link List
iProX dataset URI