PXD048384

PXD048384 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Cysteine oxidation as a regulatory mechanism of Arabidopsis plastidial NAD-dependent malate dehydrogenase
Description	Malate dehydrogenases (MDHs) catalyze a reversible NAD(P)-dependent-oxidoreductase reaction that plays an important role in central metabolism and redox homeostasis of plant cells. Recent studies suggest a moonlighting function of plastidial NAD-dependent MDH (plNAD-MDH) in plastid biogenesis, independent of its enzyme activity. Since plNAD-MDH has been known to be constitutively active, we aimed to clarify if an inactive plNAD-MDH could occur in vivo. In this study, redox-effects on activity and conformation of recombinant plNAD-MDH from Arabidopsis were investigated. We show that reduced plNAD-MDH is active, while it is inhibited upon oxidation. Interestingly, the presence of its cofactors NAD+ and NADH could prevent oxidative inhibition of plNAD-MDH. In addition, a conformational change upon oxidation could be observed via non-reducing SDS-PAGE. Both effects, its inhibition and conformational change, were reversible by re-reduction. Further investigation of single cysteine substitutions and mass spectrometry revealed that oxidation of plNAD-MDH leads to oxidation of all four cysteine residues. However, cysteine oxidation of C129 leads to inhibition of plNAD-MDH activity and oxidation of C147 induces its conformational change. In contrast, oxidation of C190 and C333 do not affect plNAD-MDH activity or structure, respectively. Taken together, our results demonstrate that plNAD-MDH activity can be reversibly inhibited, but not inactivated, by cysteine oxidation and might be co-regulated by the availability of its cofactors in vivo.
HostingRepository	jPOST
AnnounceDate	2024-04-29
AnnouncementXML	Submission_2024-04-29_02:40:49.499.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jürgen Eirich
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	unknown modification; L-methionine sulfoxide; L-cysteine sulfenic acid; L-cysteine sulfinic acid; L-cysteic acid (L-cysteine sulfonic acid)
Instrument	Orbitrap Exploris 480

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2024-01-10 05:19:14	ID requested
⏵ 1	2024-04-29 02:40:49	announced

Publication List

Dataset with its publication pending

Dataset with its publication pending

Keyword List

submitter keyword: Arabidopsis thaliana, cysteine oxidation, malate dehydrogenase, plastid, posttranslational regulation, redox

submitter keyword: Arabidopsis thaliana, cysteine oxidation, malate dehydrogenase, plastid, posttranslational regulation, redox

Contact List

Iris Finkemeier
lab head
Jürgen Eirich
contact affiliation	University of Münster - WWU - Institute for Biology and Biotechnology of Plants
dataset submitter

Full Dataset Link List

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jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST002454/