PXD048184 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Asp23-controlled cell wall imbalance promotes membrane vesicles biogenesis in Staphylococcus aureus |
Description | Membrane vesicles (MVs) are produced by species across all domains of life and have diverse physiological functions and promising applications. While the mechanisms for vesiculation in Gram-negative bacteria are well-established, the genetic determinant and regulation of MVs biogenesis in Gram-positive bacteria remain still largely unknown. Here, we demonstrate that a Q225P substitution in the alternative sigma factor SigB greatly triggers MVs production in Staphylococcus aureus strain Newman by directly repressing expression of alkaline shock protein 23, wherein the Q225P mutation hinders the specific binding of SigB to the asp23 promoter. Isogenic deletion of asp23 consistently promotes MVs formation in Newman, highlighting the critical role of both sigB and asp23 in modulating S. aureus vesiculation. While bacterial growth and cytoplasmic membrane fluidity do not be impaired, mutation of asp23 elicits a weakened cell wall and enhanced autolysis that potentially involved in LrgAB-controlled hydrolases and PSMĪ±-mediated activities, which ultimately leads to hypervesiculation in Newman. Furthermore, TEM and proteomic analysis suggest nearly identical morphology and composition, but virulence-associated factors are significantly enriched in MVs upon asp23 deletion. Overall, this study reveals novel genetic determinants and cues underlying S. aureus vesiculation, advancing the understanding of the physiology of MVs biogenesis in Gram-positive bacteria. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_06:19:50.732.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Zhu Keting |
SpeciesList | scientific name: Staphylococcus aureus (strain Newman); NCBI TaxID: 426430; |
ModificationList | No PTMs are included in the dataset |
Instrument | timsTOF Pro |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-12-31 06:02:11 | ID requested | |
1 | 2024-01-10 01:52:20 | announced | |
⏵ 2 | 2024-10-22 06:19:51 | announced | 2024-10-22: Updated project metadata. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: membrane vesicles |
Staphylococcus aureus |
SigB |
Asp23 |
proteomic analysis |
Contact List
Keting Zhu |
contact affiliation | Department of Emergency Medicine, Xinqiao Hospital, Army Medical University, 83 Xinqiao Street, Shapingba District, Chongqing 400037, China |
contact email | 406252237@qq.com |
lab head | |
Zhu Keting |
contact affiliation | Department of Emergency Medicine, Xinqiao Hospital, Army Medical University, 83 Xinqiao Street, Shapingba District, Chongqing 400037, China |
contact email | 406252237@qq.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD048184
- Label: PRIDE project
- Name: Asp23-controlled cell wall imbalance promotes membrane vesicles biogenesis in Staphylococcus aureus