PXD048024 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Molecular-Level Architecture of Chlamydomonas reinhardtii’s Glycoprotein-Rich Cell Wall |
Description | Microalgae are a renewable and promising biomass for large-20 scale biofuel, food and nutrient production. However, their efficient exploitation depends on our knowledge of the cell wall composition and organization as it can limit access to high-value molecules. Here we provide an atomic-level model of the non-crystalline and water-insoluble glycoprotein-rich cell wall of Chlamydomonas reinhardtii. Using in situ solid-state and sensitivity-enhanced nuclear magnetic 25 resonance, we reveal unprecedented details on the protein and carbohydrate composition and their nanoscale heterogeneity, as well as the presence of spatially segregated protein- and glycan-rich regions with different dynamics and hydration levels. We show that mannose-rich lower molecular-weight proteins likely contribute to the cell wall cohesion by binding to high-molecular weight protein components, and that water provides plasticity to the cell-wall architecture. The 30 structural insight exemplifies strategies used by nature to form cell walls devoid of cellulose or other glycan polymers. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_06:19:38.452.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Adnan Halim |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-12-20 21:52:08 | ID requested | |
1 | 2024-01-08 23:45:11 | announced | |
⏵ 2 | 2024-10-22 06:19:38 | announced | 2024-10-22: Updated project metadata. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: LC-MSMS, O-Man, glycoproteomics, PTM,Glycoprotein, Cell wall |
Contact List
Adnan Halim |
contact affiliation | Copenhagen Center for Glycomics (CCG) Department of Cellular and Molecular Medicine University of Copenhagen |
contact email | halim@sund.ku.dk |
lab head | |
Adnan Halim |
contact affiliation | University of Copenhagen |
contact email | halim@sund.ku.dk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/01/PXD048024 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD048024
- Label: PRIDE project
- Name: Molecular-Level Architecture of Chlamydomonas reinhardtii’s Glycoprotein-Rich Cell Wall