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PXD047799

PXD047799 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleDecrypting the molecular basis of cellular drug phenotypes by dose-resolved expression proteomics
DescriptionProteomics is making important contributions to drug discovery - from target deconvolution to mechanism of action (MoA) elucidation and the identification of biomarkers of drug response. Here, we introduce decryptE, a proteome-wide approach that measures the full dose-response characteristics of drug-induced protein expression changes which informs cellular drug MoA. Assaying 144 clinical drugs and research compounds against 8,000 proteins resulted in >1 million dose-response curves that can be interactively explored online in ProteomicsDB and a custom-built ShinyApp. Analysis of the collective data provided molecular explanations for known phenotypic drug effects and uncovered novel aspects of the MoAs of human medicines. Most notable, HDAC inhibitors potently and strongly down-regulated the T-cell receptor complex resulting in impaired human T-cell activation in-vitro and ex-vivo. This not only offers a rational explanation for the efficacy of HDAC inhibitors in certain lymphomas and autoimmune diseases but also their poor performance in treating solid tumors.
HostingRepositoryMassIVE
AnnounceDate2024-03-04
AnnouncementXMLSubmission_2024-03-04_10:10:48.975.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterStephan Eckert
SpeciesList scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; scientific name: Escherichia coli; common name: E. coli; NCBI TaxID: 562; scientific name: Pseudomonas aeruginosa; NCBI TaxID: 287; scientific name: Mus musculus; common name: house mouse; NCBI TaxID: 10090; scientific name: Arabidopsis thaliana; common name: thale cress; NCBI TaxID: 3702;
ModificationListOxidation; Carbamidomethyl; Acetyl; Methyl; Dimethyl
InstrumentOrbitrap Eclipse; Orbitrap Exploris 480
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-12-13 12:34:55ID requested
12024-03-04 10:10:49announced
Publication List
no publication
Keyword List
submitter keyword: FAIMS, micro-flow liquid chromatography, drug screen, drugs, decryptE, mode of action, dose-response, protein expression profiles, primary T cells, HDAC inhibitors, HDAC, proteomics
Contact List
Bernhard Kuster
contact affiliationTechnical University of Munich (TUM)
contact emailkuster@tum.de
lab head
Stephan Eckert
contact affiliationTechnical University Munich (TUM)
contact emailstephan.eckert@tum.de
dataset submitter
Full Dataset Link List
MassIVE dataset URI
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/v06/MSV000093659/