PXD047793 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | A hybrid DDA/DIA-PASEF based assay library for a deep proteotyping of triple-negative breast cancer |
Description | Generation of a new library of targeted mass spectrometry assays for accurate protein quantification in triple negative breast cancer (TNBC) tissues. Primary tumor tissue lysates from 105 TNBC patients treated at Masaryk Memorial Cancer Institute (MMCI) in Brno, Czech Republic, were used to generate the spectral library. This project covers raw files from data-dependent acquisition (DDA) – parallel accumulation-serial fragmentation (PASEF) measurements of 12 hydrophilic chromatography (HILIC) fractions of aliquot pool from complete set of 105 samples measured on timsTOF Pro; raw files of 16 individual samples measured in data-independent acquisition (DIA) – PASEF mode and used for hybrid library generation and for demonstrative quantitative DIA data extraction; Pulsar archive generated in Spectronaut 16.0 from 12 DDA-PASEF measurements of HILIC fractions and from 16 data-independent acquisition DIA-PASEF measurements of individual samples.
The 16 DIA-PASEF runs of individual samples used for library generation were analyzed using newest versions of Spectronaut (version 18.5) and DIA-NN (version 1.8.1) software tools in library-based setting using the newly generated library as well as in library-free setting showing library-based method to outperform the use of predicted libraries in the terms of identification numbers. |
HostingRepository | PRIDE |
AnnounceDate | 2024-05-31 |
AnnouncementXML | Submission_2024-05-31_09:44:55.055.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Pavel Bouchal |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | methylthiolated residue |
Instrument | timsTOF Pro |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-12-13 08:14:32 | ID requested | |
⏵ 1 | 2024-05-31 09:44:55 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: proteomics, mass spectrometry,data independent acquisition, assay library, triple-negative breast cancer |
Contact List
Pavel Bouchal |
contact affiliation | Masaryk University, Faculty of Science |
contact email | bouchal@chemi.muni.cz |
lab head | |
Pavel Bouchal |
contact affiliation | Masaryk University, Faculty of Science |
contact email | bouchal@chemi.muni.cz |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/05/PXD047793 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD047793
- Label: PRIDE project
- Name: A hybrid DDA/DIA-PASEF based assay library for a deep proteotyping of triple-negative breast cancer