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PXD047793

PXD047793 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleA hybrid DDA/DIA-PASEF based assay library for a deep proteotyping of triple-negative breast cancer
DescriptionGeneration of a new library of targeted mass spectrometry assays for accurate protein quantification in triple negative breast cancer (TNBC) tissues. Primary tumor tissue lysates from 105 TNBC patients treated at Masaryk Memorial Cancer Institute (MMCI) in Brno, Czech Republic, were used to generate the spectral library. This project covers raw files from data-dependent acquisition (DDA) – parallel accumulation-serial fragmentation (PASEF) measurements of 12 hydrophilic chromatography (HILIC) fractions of aliquot pool from complete set of 105 samples measured on timsTOF Pro; raw files of 16 individual samples measured in data-independent acquisition (DIA) – PASEF mode and used for hybrid library generation and for demonstrative quantitative DIA data extraction; Pulsar archive generated in Spectronaut 16.0 from 12 DDA-PASEF measurements of HILIC fractions and from 16 data-independent acquisition DIA-PASEF measurements of individual samples. The 16 DIA-PASEF runs of individual samples used for library generation were analyzed using newest versions of Spectronaut (version 18.5) and DIA-NN (version 1.8.1) software tools in library-based setting using the newly generated library as well as in library-free setting showing library-based method to outperform the use of predicted libraries in the terms of identification numbers.
HostingRepositoryPRIDE
AnnounceDate2024-05-31
AnnouncementXMLSubmission_2024-05-31_09:44:55.055.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterPavel Bouchal
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmethylthiolated residue
InstrumenttimsTOF Pro
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-12-13 08:14:32ID requested
12024-05-31 09:44:55announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: proteomics, mass spectrometry,data independent acquisition, assay library, triple-negative breast cancer
Contact List
Pavel Bouchal
contact affiliationMasaryk University, Faculty of Science
contact emailbouchal@chemi.muni.cz
lab head
Pavel Bouchal
contact affiliationMasaryk University, Faculty of Science
contact emailbouchal@chemi.muni.cz
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
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