PXD047668

PXD047668 is an original dataset announced via ProteomeXchange.

Title	CSB promotes transcription-coupled repair of DNA-protein crosslinks
Description	PxP-MS (Purification of x-linked Proteins coupled to Mass Spectrometry) was used to assess the role of CSB in DNA-protein crosslink repair. The CSB protein is a sensor that can detect stalled RNA polymerases at sites of DNA damage, thereby triggering transcription-coupled repair mechanisms. DPCs were induced in WT and CSB knock-out RPE1 cells using a pulse of formaldehyde. To identify crosslinked proteins that specifically require CSB for repair, DNA-protein crosslinks were isolated from cells using PxP either directly after formaldehyde exposure or following a chase in drug-free media and identified by mass spectrometry.
HostingRepository	PRIDE
AnnounceDate	2024-02-15
AnnouncementXML	Submission_2024-02-15_09:08:28.156.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Fatih Aygenli
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2023-12-09 06:34:03	ID requested
⏵ 1	2024-02-15 09:08:28	announced

Dataset with its publication pending

submitter keyword: Cockayne syndrome, formaldehyde, DNA damage repair, DNA-protein crosslinks, CSB

Charo Robles
contact affiliation	System Chronobiology Institute for Medical Psychology Ludwig-Maximilians Universitat Muenchen Germany (lab head)
contact email	charo.robles@med.uni-muenchen.de
lab head
Fatih Aygenli
contact affiliation	LMU - Muenchen
contact email	fatih.aygenli@med.uni-muenchen.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/02/PXD047668

PRIDE project URI

• PRIDE
  1. PXD047668
     1. Label: PRIDE project
     2. Name: CSB promotes transcription-coupled repair of DNA-protein crosslinks