PXD047537 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Synthetically primed adaptation of Pseudomonas putida to a non-native substrate D-xylose |
Description | To broaden microbial cell factories´ substrate scope towards renewable substrates, rational genetic interventions are often combined with adaptive laboratory evolution (ALE). However, comprehensive studies enabling a holistic understanding of adaptation processes primed by detailed knowledge of metabolism remain scarce, especially for non-model organisms. The industrial workhorse Pseudomonas putida was engineered to utilize the non-native sugar D-xylose, but its assimilation into the bacterial biochemical network via the exogenous pathway remained unresolved. Here, we elucidated the xylose metabolism and established a foundation for further engineering followed by ALE. By de-repressing native glycolysis, we unlocked the route for xylose-derived carbon and obtained a strain with a substantially reduced lag phase on xylose. We then enhanced the pentose phosphate pathway in two lag-shortened strains and allowed ALE to fine-tune the rewired metabolism. Following the metabolism tuning, we employed multi-level analysis that provided unique insights into the parallel paths of bacterial adaptation to the non-native carbon source. |
HostingRepository | PRIDE |
AnnounceDate | 2024-03-06 |
AnnouncementXML | Submission_2024-03-06_01:34:41.409.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Katerina Hanakova |
SpeciesList | scientific name: Pseudomonas putida KT2440; NCBI TaxID: 160488; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Orbitrap Exploris 480 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-12-05 05:29:32 | ID requested | |
⏵ 1 | 2024-03-06 01:34:41 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Pseudomonas putida, LC-MS/MS |
Contact List
Zbynek Zdrahal |
contact affiliation | Proteomics Core Facility and Research Group Proteomics, CEITEC-MU, Masaryk University, Brno, Czech Republic |
contact email | zdrahal@sci.muni.cz |
lab head | |
Katerina Hanakova |
contact affiliation | CEITEC MU |
contact email | katerina.hanak@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD047537
- Label: PRIDE project
- Name: Synthetically primed adaptation of Pseudomonas putida to a non-native substrate D-xylose