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PXD047537

PXD047537 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleSynthetically primed adaptation of Pseudomonas putida to a non-native substrate D-xylose
DescriptionTo broaden microbial cell factories´ substrate scope towards renewable substrates, rational genetic interventions are often combined with adaptive laboratory evolution (ALE). However, comprehensive studies enabling a holistic understanding of adaptation processes primed by detailed knowledge of metabolism remain scarce, especially for non-model organisms. The industrial workhorse Pseudomonas putida was engineered to utilize the non-native sugar D-xylose, but its assimilation into the bacterial biochemical network via the exogenous pathway remained unresolved. Here, we elucidated the xylose metabolism and established a foundation for further engineering followed by ALE. By de-repressing native glycolysis, we unlocked the route for xylose-derived carbon and obtained a strain with a substantially reduced lag phase on xylose. We then enhanced the pentose phosphate pathway in two lag-shortened strains and allowed ALE to fine-tune the rewired metabolism. Following the metabolism tuning, we employed multi-level analysis that provided unique insights into the parallel paths of bacterial adaptation to the non-native carbon source.
HostingRepositoryPRIDE
AnnounceDate2024-03-06
AnnouncementXMLSubmission_2024-03-06_01:34:41.409.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterKaterina Hanakova
SpeciesList scientific name: Pseudomonas putida KT2440; NCBI TaxID: 160488;
ModificationListiodoacetamide derivatized residue
InstrumentOrbitrap Exploris 480
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-12-05 05:29:32ID requested
12024-03-06 01:34:41announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Pseudomonas putida, LC-MS/MS
Contact List
Zbynek Zdrahal
contact affiliationProteomics Core Facility and Research Group Proteomics, CEITEC-MU, Masaryk University, Brno, Czech Republic
contact emailzdrahal@sci.muni.cz
lab head
Katerina Hanakova
contact affiliationCEITEC MU
contact emailkaterina.hanak@gmail.com
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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