PXD047178

PXD047178 is an original dataset announced via ProteomeXchange.

Title	DPP8/9 processing of human AK2 unmasks an IAP binding motif
Description	AK2 is n-terminally processed by methionine aminopeptidases, N-terminal acetylation and DDP8/9 to become an active protein which is then sorted in the mitochondrial intermembrane space. We used peptide pull-downs of free and acetylated N-terminal peptides of AK2 from the different processing steps to identify binding partners by MS. We identified the inhibitor of apoptosis E3 ligase proteins (IAPs; BIRC2, 3, 6 and XIAP) as new binding partners for processed AK2 and show that they are responsible for AK2 degradation in the cytosol.
HostingRepository	PRIDE
AnnounceDate	2025-03-13
AnnouncementXML	Submission_2025-03-13_09:14:49.427.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tanja Bange
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2023-11-22 12:36:43	ID requested
⏵ 1	2025-03-13 09:14:49	announced

Dataset with its publication pending

submitter keyword: DDP8/9 cleavage, peptide pull-downs, mass spectrometry,N-terminal processing, N-terminal acetlyation

Tanja Bange
contact affiliation	Institute of Medical Psychology and Biomedical Center (BMC), Faculty of Medicine, LMU Munich, Germany
contact email	tanja.bange@med.uni-muenchen.de
lab head
Tanja Bange
contact affiliation	LMU Munich
contact email	tanja.bange@med.uni-muenchen.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/03/PXD047178

PRIDE project URI

• PRIDE
  1. PXD047178
     1. Label: PRIDE project
     2. Name: DPP8/9 processing of human AK2 unmasks an IAP binding motif