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PXD047097

PXD047097 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleThe long-chain flavodoxin FldX1 improves the biodegradation of 4-hydroxyphenylacetate and 3-hydroxyphenylacetate and counteracts the oxidative stress associated to aromatic catabolism in Paraburkholderia xenovorans
DescriptionBacterial aromatic degradation may cause oxidative stress. The long-chain flavodoxin FldX1 of Paraburkholderia xenovorans LB400 counteracts reactive oxygen species (ROS). The aim of this study was to evaluate the protective role of FldX1 in P. xenovorans LB400 during the degradation of 4-hydroxyphenylacetate (4-HPA) and 3-hydroxyphenylacetate (3-HPA). Functionality of FldX1 was assessed by P. xenovorans p2-fldX1 that overexpresses FldX1. The effects of FldX1 on P. xenovorans were studied measuring growth on hydroxyphenylacetates, degradation of 4-HPA and 3-HPA, and ROS formation. The effects of hydroxyphenylacetates on the proteome (LC–MS/MS) and gene expression (qRT-PCR) were quantified. Bioaugmentation with strain p2-fldX1 of 4-HPA-polluted soil was assessed, measuring aromatic degradation (HPLC), 4-HPA-degrading bacteria, and plasmid stability.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_06:34:48.894.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterDaniela Zuehlke
SpeciesList scientific name: Paraburkholderia xenovorans LB400; NCBI TaxID: 266265;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-11-20 05:32:21ID requested
12024-03-27 04:29:25announced
22024-10-22 06:34:49announced2024-10-22: Updated project metadata.
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: flavodoxin
long-chain flavodoxin
Paraburkholderia xenovorans LB400
Oxidative stress
Hydroxyphenylacetate
Proteome
Contact List
Katharina Riedel
contact affiliationDepartment of Microbial Physiology and Molecular Biology, Institute of Microbiology, University of Greifswald
contact emailriedela@uni-greifswald.de
lab head
Daniela Zuehlke
contact affiliationUniversity of Greifswald, Institute of Microbiology
contact emailzuehlke@uni-greifswald.de
dataset submitter
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Dataset FTP location
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