PXD047087
PXD047087 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Comprehensive identification of Ubiquitin-like 3 (UBL3)-interacting proteins in mouse brain |
Description | Each protein extract from the cortex of Ubl3+/+ and Ubl3Tg/+ male mice (9-10-week-old, n=3, 3) was incubated with 30 μL of NeutrAvidin Agarose (ThermoFisher, 29202) for 18 h at 4 °C and subjected to mass spectrometry using previously reported methods (Ageta et al., Nat Commun 2018, PMID: 30258067). The peptides were analyzed by liquid chromatography/mass spectrometry (LC/MS) using an Orbitrap Fusion mass spectrometer coupled to an Easy-nLC 1000 using a EASY-Spray ES900 column system (ThermoFisher, 75 μm x 150 mm, particle size 3 μm). Mass spectrometry raw files is proceeded using Proteome Discoverer version 2.4 (ThermoFisher) and a local MASCOT server (version 2.6.2; Matrix Science). MS/MS data were searched against Mus musculus (SwissProt TaxID=10090_and_subtaxonomies) (v2017-10-25). |
HostingRepository | jPOST |
AnnounceDate | 2024-11-20 |
AnnouncementXML | Submission_2024-11-19_07:00:07.317.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD047087 |
ReviewLevel | Non peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Hiroshi Ageta |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide; alpha-amino acetylated residue |
Instrument | LTQ Orbitrap |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2023-11-20 01:22:03 | ID requested | |
⏵ 1 | 2024-11-19 07:00:07 | announced |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: UBL3 |
Contact List
Kunihiro Tsuchida | |
---|---|
lab head | |
Hiroshi Ageta | |
contact affiliation | Fujita Health University |
dataset submitter |
Full Dataset Link List
jPOST dataset URI |
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST002396/ |