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PXD047009

PXD047009 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleExosomes define a local and systemic communication network in healthy pancreas and in pancreatic ductal adenocarcinoma
DescriptionPancreatic ductal adenocarcinoma (PDAC) is a deadly disease. Understanding its biology is crucial for finding effective therapies. Exosomes have been implicated in cancer, but their behavior in living systems remains poorly understood. We aimed to map the spatiotemporal distribution of exosomes from both healthy pancreas and PDAC to determine their biological significance. To achieve this, we developed a genetically engineered mouse model (ExoBow) to trace spontaneous exosomes communication. Within the PDAC microenvironment, cancer cells establish preferential communication routes with cancer associated fibroblasts and endothelial cells. The latter is a conserved event in the healthy pancreas. Inhibiting exosomes secretion in both scenarios significantly enhanced angiogenesis, underscoring the contribution of exosomes to the vascularization of the organ and to cancer. Inter-organ communication is significantly increased in PDAC, and the thymus is a sustained target in both contexts. PDAC cells also communicate with the kidneys and lungs, and the healthy pancreas with bone-marrow, brain, and intestines. In sum, we found that exosomes mediate an organized communication network in vivo that controls angiogenesis locally, both in the healthy pancreas and PDAC, and that targets the thymus also in both conditions, unravelling their potential role in central immune surveillance and anti-tumor immune response.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_06:17:22.450.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterHugo Osorio
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListiodoacetamide derivatized residue
InstrumentOrbitrap Eclipse
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-11-16 00:40:48ID requested
12023-12-12 10:47:13announced
22024-10-22 06:17:22announced2024-10-22: Updated project metadata.
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: exosomes,Pancreatic cancer, intercellular communication, tumor microenvironment
Contact List
Sonia A. Melo
contact affiliationi3S - Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Portugal. Departamento de Patologia, Faculdade de Medicina, Universidade do Porto, Portugal. P.CCC Porto Comprehensive Cancer Center Raquel Seruca, Portugal.
contact emailsmelo@i3s.up.pt
lab head
Hugo Osorio
contact affiliationi3S - Institute for Research and Innovation in Health, University of Porto
contact emailhosorio@i3s.up.pt
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
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