PXD046448 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | The CDK Pho85 inhibits Whi7 Start repressor to promote cell cycle entry in budding yeast |
Description | Pho85 is a multifunctional CDK that signals to the cell when environmental conditions are favorable. It has been connected to cell cycle control, mainly in Start where it promotes the G1/S transition. Here we describe that the Start repressor Whi7 is a key target of Pho85 in the regulation of cell cycle entry. The phosphorylation of Whi7 by Pho85 inhibits the repressor and explains most of the contribution of the CDK in the activation of Start. Mechanistically, Pho85 down-regulates Whi7 protein levels through the control Whi7 protein stability and WHI7 gene transcription. Whi7 phosphorylation by Pho85 also restrains the intrinsic ability of Whi7 to associate with promoters. Furthermore, although Whi5 is the main Start repressor in normal cycling cells, in the absence of Pho85 Whi7 becomes the major repressor leading to G1 arrest. Overall, our results reveal a novel mechanism by which Pho85 promotes Start through the regulation of the Whi7 repressor at multiple levels, which may confer to Whi7 a functional specialization to connect the response to adverse conditions with the cell cycle control. |
HostingRepository | PRIDE |
AnnounceDate | 2023-12-13 |
AnnouncementXML | Submission_2023-12-13_03:06:38.180.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Luz Valero |
SpeciesList | scientific name: Saccharomyces cerevisiae; NCBI TaxID: NCBITaxon:4932; scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932; |
ModificationList | phosphorylated residue |
Instrument | Bruker Daltonics instrument model |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-10-27 05:21:25 | ID requested | |
⏵ 1 | 2023-12-13 03:06:38 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: dda-PASEF,phospho proteomics |
Contact List
Juan Carlos Igual |
contact affiliation | Institut de Biotecnologia i Biomedicina (BIOTECMED) and Departament de Bioquímica i Biologia Molecular, Universitat de València, 46100 Burjassot, Spain |
contact email | Juan.C.Igual@uv.es |
lab head | |
Luz Valero |
contact affiliation | Universitat de Valencia |
contact email | mluz.valero@uv.es |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
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[ - ]
- PRIDE
- PXD046448
- Label: PRIDE project
- Name: The CDK Pho85 inhibits Whi7 Start repressor to promote cell cycle entry in budding yeast