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PXD046411

PXD046411 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleMapping the immunopeptidome of several SARS-CoV-2 antigens across common HLA haplotypes
DescriptionMost COVID-19 vaccines have been designed to elicit immunity against the SARS-CoV-2 Spike protein. However, the repeated occurrence of new strains harbouring Spike protein mutations demonstrates ready immune evasion by the SARS-CoV-2 virus and the pressing need to develop more broadly targeting COVID-19 vaccines. To facilitate this, we used mass spectrometry to identify immunopeptides that are derived from seven structural and non-structural SARS-CoV-2 proteins that are relatively conserved across viral strains (N, E, Nsp1, Nsp4, Nsp5, Nsp8, Nsp9) and presented by prevalent Human Leukocyte Antigen (HLA) class I and class II molecules. Two different B-lymphoblastoid cell lines were chosen to map immunopeptidomes covering some of the major HLA types across the global human population. We used DNA plasmid transfection and direct antigen delivery approaches to sample different antigens. We found 248 unique HLA class I and HLA class II bound peptides with 12 derived from E, 71 from N, 28 from Nsp1, 19 from Nsp4, 73 from Nsp8 and 45 peptides derived from Nsp9. T cell responses were tested for 56 of the detected peptides and we show robust CD8+ and CD4+ T cell responses against several peptides from the N, E and Nsp9 proteins. Results from this study will aid the development of next-generation COVID vaccines targeting epitopes from across a number of SARS-CoV-2 proteins.
HostingRepositoryMassIVE
AnnounceDate2024-08-03
AnnouncementXMLSubmission_2024-08-03_17:46:34.963.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterA. Braun
SpeciesList scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; scientific name: Severe acute respiratory syndrome coronavirus 2; NCBI TaxID: 2697049;
ModificationListOxidation; Acetyl; Deamidated
InstrumenttimsTOF Pro
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-10-26 03:34:56ID requested
12024-08-03 17:46:35announced
Publication List
no publication
Keyword List
submitter keyword: B lymphoblastoid cell line, SARS-CoV-2, COVID-19
Contact List
Anthony Wayne Purcell
contact affiliationDeputy Head (Research), Department of Biochemistry and Molecular Biology Head Immunoproteomics Laboratory Infection and Immunity Program Monash Biomedicine Discovery Institute Monash University, Clayton Campus Clayton 3800 Victoria Australia
contact emailanthony.purcell@monash.edu
lab head
A. Braun
contact affiliationMonash
contact emailassolbraun@web.de
dataset submitter
Full Dataset Link List
MassIVE dataset URI
Dataset FTP location
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