PXD046404

PXD046404 is an original dataset announced via ProteomeXchange.

Title	In-depth Profiling of 4-hydroxy-2-nonenal Modification via Reversible Thiazolidine Chemistry
Description	Protein modification by lipid-derived electrophiles (LDEs) associates with various signaling pathways. Among these LDEs, HNE, is the most toxic and proteins modification of HNE have been linked to various diseases, including Alzheimer's and Parkinson's. However, due to their low abundance, in-depth profiling of HNEs modifications still presents challenges. This study introduces a novel strategy utilizing reversible thiazolidine chemistry to selectively capture HNE-modified proteins and palladium-mediated cleavage reaction to release them. Thousands of HNE-modified sites in different cell lines were identified. Combined with ABPP, we discovered a set of HNE-sensitive sites, offers a new tool for studying LDEs modifications in proteomes.
HostingRepository	iProX
AnnounceDate	2023-10-26
AnnouncementXML	Submission_2023-10-25_18:51:47.743.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jun Wang
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2023-10-25 18:51:36	ID requested
⏵ 1	2023-10-25 18:51:48	announced

Dataset with its publication pending

submitter keyword: HNE

Haojie Lu
contact affiliation	Shanghai Cancer Center and Institutes of Biomedical Sciences, Fudan University
contact email	luhaojie@fudan.edu.cn
lab head
Jun Wang
contact affiliation	Department of Chemistry and Shanghai Cancer Center, Fudan University
contact email	21110220037@m.fudan.edu.cn
dataset submitter

iProX dataset URI