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PXD046385

PXD046385 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleRibosomal Protein SA (RPSA) is required for localized translation and sarcomere maintenance
DescriptionCardiomyocyte sarcomeres contain localized ribosomes, but the factors responsible for their localization and the significance of localized translation are unknown. Using proximity labeling, we identified Ribosomal Protein SA (RPSA) as a Z-line protein. In cultured cardiomyocytes, the loss of RPSA led to impaired local protein translation and reduced sarcomere integrity. By employing CAS9 expression mice along with adeno-associated viruses expressing CRE recombinase and single-guide RNAs targeting Rpsa, we knocked out RPSA in vivo and observed mis-localization of ribosomes and diminished local translation. These genetic mosaic mice with RPSA knockout in a subset of cardiomyocytes developed dilated cardiomyopathy, featuring atrophy of RPSA-deficient cardiomyocytes, compensatory hypertrophy of unaffected cardiomyocytes, left ventricular dilation, and impaired contractile function. We demonstrate that RPSA C-terminal domain is sufficient for localization Z-lines. These findings highlight RPSA as a ribosomal factor responsible for ribosome localization to the Z-line, facilitating local translation and sarcomere maintenance.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_06:43:46.486.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterTamar Ziv
SpeciesList scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationListiodoacetamide derivatized residue
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-10-25 06:50:21ID requested
12024-06-04 09:52:12announced
22024-10-22 06:43:46announced2024-10-22: Updated project metadata.
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Ribosome, Localized translation, proximity labeling, Sarcomere, Z-line
Contact List
Izhak Kehat
contact affiliation2The Rappaport Institute and the Bruce Rappaport Faculty of Medicine, Technion – Israel Institute of Technology, Israel.
contact emailikehat@technion.ac.il
lab head
Tamar Ziv
contact affiliationTechnion
contact emailtamarz@technion.ac.il
dataset submitter
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Dataset FTP location
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PRIDE project URI
Repository Record List
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