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PXD046382

PXD046382 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleCross-link assisted spatial proteomics to map sub-organelle proteomes and membrane protein topology
DescriptionThe specific functions of cellular organelles and sub-compartments depend on their protein content, which can be characterized by spatial proteomics approaches. However, many spatial proteomics methods are limited in their ability to resolve organellar sub-compartments, profile multiple sub-compartments in parallel, and/or characterize membrane-associated proteomes. Here, we develop a cross-linking assisted spatial proteomics (CLASP) strategy that addresses these shortcomings. Using human mitochondria as a model system, we show that CLASP can elucidate spatial proteomes of all mitochondrial sub-compartments and provide topological insight into the mitochondrial membrane proteome in a single experiment. Biochemical and imaging-based follow-up studies demonstrate that CLASP allows discovering mitochondria-associated proteins and revising previous protein sub-compartment localization and membrane topology data. This study extends the scope of cross-linking mass spectrometry beyond protein structure and interaction analysis towards spatial proteomics, establishes a method for concomitant profiling of sub-organelle and membrane proteomes, and provides a resource for mitochondrial spatial biology
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_06:31:25.831.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterKerem Akkaya
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-10-25 05:57:26ID requested
12024-02-21 08:02:17announced
22024-10-22 06:31:26announced2024-10-22: Updated project metadata.
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: synaptic vesicles,XL-MS, mitochondria, spatial proteomics, DSBSO
Contact List
Fan Liu
contact affiliationDepartment of Structural Biology, Leibniz-Forschungsinstitut für Molekulare Pharmakologie (FMP)
contact emailFLiu@fmp-berlin.de
lab head
Kerem Akkaya
contact affiliationLeibniz-Forschungsinstitut für Molekulare Pharmakologie
contact emailakkaya@fmp-berlin.de
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
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