PXD046358 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | The virulence regulator VirB from Shigella flexneri uses a CTPdependent switch mechanism to activate gene expression |
Description | The transcriptional antisilencer VirB acts as a master regulator of virulence gene expression in the human pathogen Shigella flexneri. It binds defined sequences (virS) upstream of VirB-dependent promoters and counteracts their silencing by the nucleoid-organizing protein H-NS. However, its precise mode of action remains unclear. Notably, VirB is not a classical transcription factor but related to DNA partitioning pro- teins of the ParB family, which have recently been recognized as DNA-sliding clamps using CTP binding and hydrolysis to control their DNA entry gate. Here, we show that VirB binds CTP, embraces DNA in a clamp-like fashion upon its CTP-dependent loading at virS sites and slides laterally on DNA after clamp closure. Mutations that prevent CTP binding block the loading of VirB clamps in vitro and the formation of VirB nucleoprotein complexes in vivo. Thus, VirB represents a CTP-dependent molecular switch that uses a loading-and-sliding mechanism to control transcription during bacterial pathogenesis. |
HostingRepository | PRIDE |
AnnounceDate | 2023-12-06 |
AnnouncementXML | Submission_2023-12-06_11:42:51.904.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Timo Glatter |
SpeciesList | scientific name: Shigella flexneri 5a; NCBI TaxID: 424718; |
ModificationList | No PTMs are included in the dataset |
Instrument | Orbitrap Exploris 480 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-10-24 09:10:32 | ID requested | |
⏵ 1 | 2023-12-06 11:42:52 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: ParABS system,Shigella flexneri, type III secretion |
Contact List
Timo Glatter |
contact affiliation | Max Planck Insitute for Terrestrial Microbiology Karl-von-Frisch Str. 10 35043 Marburg Germany |
contact email | timo.glatter@mpi-marburg.mpg.de |
lab head | |
Timo Glatter |
contact affiliation | Max Planck Institute for Terrestrial Microbiology Marburg |
contact email | timo.glatter@mpi-marburg.mpg.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD046358
- Label: PRIDE project
- Name: The virulence regulator VirB from Shigella flexneri uses a CTPdependent switch mechanism to activate gene expression