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PXD046358

PXD046358 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleThe virulence regulator VirB from Shigella flexneri uses a CTPdependent switch mechanism to activate gene expression
DescriptionThe transcriptional antisilencer VirB acts as a master regulator of virulence gene expression in the human pathogen Shigella flexneri. It binds defined sequences (virS) upstream of VirB-dependent promoters and counteracts their silencing by the nucleoid-organizing protein H-NS. However, its precise mode of action remains unclear. Notably, VirB is not a classical transcription factor but related to DNA partitioning pro- teins of the ParB family, which have recently been recognized as DNA-sliding clamps using CTP binding and hydrolysis to control their DNA entry gate. Here, we show that VirB binds CTP, embraces DNA in a clamp-like fashion upon its CTP-dependent loading at virS sites and slides laterally on DNA after clamp closure. Mutations that prevent CTP binding block the loading of VirB clamps in vitro and the formation of VirB nucleoprotein complexes in vivo. Thus, VirB represents a CTP-dependent molecular switch that uses a loading-and-sliding mechanism to control transcription during bacterial pathogenesis.
HostingRepositoryPRIDE
AnnounceDate2023-12-06
AnnouncementXMLSubmission_2023-12-06_11:42:51.904.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterTimo Glatter
SpeciesList scientific name: Shigella flexneri 5a; NCBI TaxID: 424718;
ModificationListNo PTMs are included in the dataset
InstrumentOrbitrap Exploris 480
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-10-24 09:10:32ID requested
12023-12-06 11:42:52announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: ParABS system,Shigella flexneri, type III secretion
Contact List
Timo Glatter
contact affiliationMax Planck Insitute for Terrestrial Microbiology Karl-von-Frisch Str. 10 35043 Marburg Germany
contact emailtimo.glatter@mpi-marburg.mpg.de
lab head
Timo Glatter
contact affiliationMax Planck Institute for Terrestrial Microbiology Marburg
contact emailtimo.glatter@mpi-marburg.mpg.de
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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