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PXD046333

PXD046333 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleRemodeling of the gastric environment in Helicobacter pylori-induced atrophic gastritis
DescriptionHelicobacter pylori colonization of the human stomach is a strong risk factor for gastric cancer. To investigate H. pylori-induced gastric molecular alterations, we used a Mongolian gerbil model of gastric carcinogenesis. Histologic evaluation revealed varying levels of atrophic gastritis (a premalignant condition characterized by parietal and chief cell loss) in H. pylori-infected animals, and transcriptional profiling revealed a loss of markers for these cell types. We then assessed the spatial distribution and relative abundance of proteins in the gastric tissues using imaging mass spectrometry and liquid chromatography with tandem mass spectrometry (LC-MS/MS). We detected striking differences in protein content of corpus and antrum tissues. 492 proteins were preferentially localized to the corpus in uninfected animals. The abundance of 91 of these proteins was reduced in H. pylori-infected corpus tissues exhibiting atrophic gastritis compared to infected corpus tissues with non-atrophic gastritis or uninfected corpus tissues; these included numerous proteins with metabolic functions. Fifty proteins localized to the corpus in uninfected animals were diffusely delocalized throughout the stomach in infected tissues with atrophic gastritis; these included numerous proteins with roles in protein processing. Corresponding alterations were not detected in animals infected with a H. pylori ∆cagT mutant (lacking Cag type IV secretion system activity). These results indicate that H. pylori can cause loss of proteins normally localized to the gastric corpus as well as diffuse delocalization of corpus-specific proteins, resulting in marked changes in the normal gastric molecular partitioning into distinct corpus and antrum regions.
HostingRepositoryPRIDE
AnnounceDate2023-12-04
AnnouncementXMLSubmission_2023-12-04_12:32:42.747.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterHayes McDonald
SpeciesList scientific name: Meriones unguiculatus; NCBI TaxID: 10047;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Exploris 480
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-10-23 12:01:33ID requested
12023-12-04 12:32:43announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: premalignant, proteomics,Helicobacter pylori, inflammation, gastric cancer, biomarkers
Contact List
Timothy Cover
contact affiliationProfessor of Medicine Professor of Pathology, Microbiology and Immunology Vanderbilt University School of Medicine
contact emailtimothy.l.cover@vumc.org
lab head
Hayes McDonald
contact affiliationVanderbilt University
contact emailhayes.mcdonald@vanderbilt.edu
dataset submitter
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Dataset FTP location
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