PXD046240
PXD046240 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Shotgun proteomics of thyroid carcinoma exosomes: insight into the role of exosomal proteins in carcinogenesis and thyroid homeostasis |
| Description | The transport of molecules via exosomes is one of the factors involved in cancer development, and transferred molecules can serve as specific tumor biomarkers. The aim of the study was the LC MS/MS proteomic analysis of exosomes released by FTC and 8305C thyroid cancer cell lines, and reference Nthy-ori 3-1 normal thyroid follicular cells. A total of 1769 unique proteins were identified in the exosome samples. For exosomes derived from Nthy ori 3 1 cell the highest number of 1504 proteins was identified, while exosomes from thyroid carcinomas FTC and 8305C cell lines had 730 and 1304 identified proteins, respectively. For the identified proteins, gene ontology analysis was performed in terms of their cellular location, molecular function and involvement in biological processes. For proteins that only appeared in tumor derived FTC- and 8305C-derived exosomes, enriched categories were related to cancer progression and included cell adhesion and positive regulation of cell migration. Also, proteins related to protein N linked glycosylation, drug resistance, and cell response to NK and T cell cytotoxicity were among those unique proteins. Finally label free quantification (LFQ) was performed for identified differentially expressed proteins between all possible group parings. For exosomes from thyroid cancer cells, the most differentiating proteins included collagen alpha 2(I) chain, tenascin, matrix metalloproteinase 1, Interstitial collagenase and C type lectin domain family 11 member A. The obtained results broadened the knowledge about the role of exosomal proteins in thyroid cancer and indicated potential biomarkers for further evaluation in clinical conditions. |
| HostingRepository | MassIVE |
| AnnounceDate | 2024-11-19 |
| AnnouncementXML | Submission_2024-11-19_05:14:09.904.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Urszula Jankowska |
| SpeciesList | scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; |
| ModificationList | Acetyl; Oxidation; Carbamidomethyl |
| Instrument | Q Exactive |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2023-10-19 06:28:32 | ID requested | |
| ⏵ 1 | 2024-11-19 05:14:10 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: biomarkers, exosomes, thyroid cancer |
Contact List
| Malgorzata Przybylo | |
|---|---|
| contact affiliation | Department of Glycoconjugate Biochemistry, Institute of Zoology and Biomedical Research, Faculty of Biology, Jagiellonian University |
| contact email | malgorzata.przybylo@uj.edu.pl |
| lab head | |
| Urszula Jankowska | |
| contact affiliation | Jagiellonian University |
| contact email | urszula.jankowska@uj.edu.pl |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/v06/MSV000093152/ |
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