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PXD046229

PXD046229 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleFully defined NGN2 neuron protocol reveals diverse signatures of neuronal maturation
DescriptionNGN2-driven iPSC-to-neuron conversion is a popular method for human neurological disease modeling. In this study, we report a fully standardized approach for generating functional excitatory cortical neurons. This method utilizes clonal, targeted-engineered iPSC lines and employs fully defined reagents. We demonstrate that our protocol generates consistent excitatory cortical neurons at scale. Comprehensive temporal omics, electrophysiological, and morphological profilings indicate the continued progression of neuronal maturation for at least 150 days. Deep quantitative characterizations through transcriptomic, imaging, and functional assays reveal coordinated actions of multiple pathways driving neuronal maturation. The neurons express the majority of the key risk genes in Alzheimer's disease, Parkinson's disease, and autism, demonstrating the relevance of our protocol in modeling human neurological disorders. This well-defined method, profiling data, and functional characterization provide a solid and reliable framework for developing human in vitro neuronal models for disease modeling.
HostingRepositoryMassIVE
AnnounceDate2024-09-20
AnnouncementXMLSubmission_2024-09-20_08:39:45.288.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMeena Choi
SpeciesList scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentOrbitrap Eclipse
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-10-18 23:29:15ID requested
12024-09-20 08:39:45announced
Publication List
no publication
Keyword List
submitter keyword: TMT, iPSC, NGN2, DDA
Contact List
Claire G. Jeong
contact affiliationGenentech
contact emailjeongc2@gene.com
lab head
Meena Choi
contact affiliationGenentech
contact emailchoimeena.ijae@gmail.com
dataset submitter
Full Dataset Link List
MassIVE dataset URI
Dataset FTP location
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