PXD046229
PXD046229 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Fully defined NGN2 neuron protocol reveals diverse signatures of neuronal maturation |
Description | NGN2-driven iPSC-to-neuron conversion is a popular method for human neurological disease modeling. In this study, we report a fully standardized approach for generating functional excitatory cortical neurons. This method utilizes clonal, targeted-engineered iPSC lines and employs fully defined reagents. We demonstrate that our protocol generates consistent excitatory cortical neurons at scale. Comprehensive temporal omics, electrophysiological, and morphological profilings indicate the continued progression of neuronal maturation for at least 150 days. Deep quantitative characterizations through transcriptomic, imaging, and functional assays reveal coordinated actions of multiple pathways driving neuronal maturation. The neurons express the majority of the key risk genes in Alzheimer's disease, Parkinson's disease, and autism, demonstrating the relevance of our protocol in modeling human neurological disorders. This well-defined method, profiling data, and functional characterization provide a solid and reliable framework for developing human in vitro neuronal models for disease modeling. |
HostingRepository | MassIVE |
AnnounceDate | 2024-09-20 |
AnnouncementXML | Submission_2024-09-20_08:39:45.288.xml |
DigitalObjectIdentifier | |
ReviewLevel | Non peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Meena Choi |
SpeciesList | scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Orbitrap Eclipse |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2023-10-18 23:29:15 | ID requested | |
⏵ 1 | 2024-09-20 08:39:45 | announced |
Publication List
no publication |
Keyword List
submitter keyword: TMT, iPSC, NGN2, DDA |
Contact List
Claire G. Jeong | |
---|---|
contact affiliation | Genentech |
contact email | jeongc2@gene.com |
lab head | |
Meena Choi | |
contact affiliation | Genentech |
contact email | choimeena.ijae@gmail.com |
dataset submitter |
Full Dataset Link List
MassIVE dataset URI |
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/v06/MSV000093150/ |