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PXD046120

PXD046120 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleThe deubiquitinase cofactor UAF1 interacts with USP1 and plays an essential role in spermiogenesis
DescriptionSpermiogenesis defines the final phase of male germ cell differentiation. Multiple deubiquitinating enzymes have been linked to spermiogenesis, yet the impacts of deubiquitination on spermiogenesis remain poorly characterized. Here, we investigated the function of UAF1 in mouse spermiogenesis and male fertility. We selectively deleted Uaf1 in premeiotic germ cells using Stra8-Cre knock-in mouse strain (Uaf1 sKO), and found that Uaf1 is essential for spermiogenesis and male fertility. We found that UAF1 interacts and colocalizes with USP1 in testes. Conditional knockout of Uaf1 in testes results in disturbed expression and localization of USP1, suggesting that UAF1 regulates spermiogenesis through the function of the deubiquitinating enzyme USP1. We used tandem mass tag-based proteomics to identify differentially expressed proteins and potential underlying mechanisms, and found that conditional knockout Uaf1 in testes results in reduced levels of proteins essential for spermiogenesis. Thus, the UAF1/USP1 deubiquitinase complex is essential for normal spermiogenesis by regulating the levels of spermiogenesis-related proteins.
HostingRepositoryPRIDE
AnnounceDate2024-02-07
AnnouncementXMLSubmission_2024-02-07_00:15:00.159.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterZiqi Wang
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListNo PTMs are included in the dataset
InstrumentLTQ Orbitrap Elite
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-10-13 06:19:45ID requested
12024-02-07 00:15:00announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: UAF1;USP1;male infertility;spermiogenesis;deubiquitylation
Contact List
Ziqi Wang
contact affiliationCenter for Reproductive Medicine, Shandong University, Jinan, Shandong 250012, China,
contact emailwangziqi1110@126.com
lab head
Ziqi Wang
contact affiliationShandong University
contact emailwangziqi1110@126.com
dataset submitter
Full Dataset Link List
Dataset FTP location
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