PXD046120 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | The deubiquitinase cofactor UAF1 interacts with USP1 and plays an essential role in spermiogenesis |
Description | Spermiogenesis defines the final phase of male germ cell differentiation. Multiple deubiquitinating enzymes have been linked to spermiogenesis, yet the impacts of deubiquitination on spermiogenesis remain poorly characterized. Here, we investigated the function of UAF1 in mouse spermiogenesis and male fertility. We selectively deleted Uaf1 in premeiotic germ cells using Stra8-Cre knock-in mouse strain (Uaf1 sKO), and found that Uaf1 is essential for spermiogenesis and male fertility. We found that UAF1 interacts and colocalizes with USP1 in testes. Conditional knockout of Uaf1 in testes results in disturbed expression and localization of USP1, suggesting that UAF1 regulates spermiogenesis through the function of the deubiquitinating enzyme USP1. We used tandem mass tag-based proteomics to identify differentially expressed proteins and potential underlying mechanisms, and found that conditional knockout Uaf1 in testes results in reduced levels of proteins essential for spermiogenesis. Thus, the UAF1/USP1 deubiquitinase complex is essential for normal spermiogenesis by regulating the levels of spermiogenesis-related proteins. |
HostingRepository | PRIDE |
AnnounceDate | 2024-02-07 |
AnnouncementXML | Submission_2024-02-07_00:15:00.159.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Ziqi Wang |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | No PTMs are included in the dataset |
Instrument | LTQ Orbitrap Elite |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-10-13 06:19:45 | ID requested | |
⏵ 1 | 2024-02-07 00:15:00 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: UAF1;USP1;male infertility;spermiogenesis;deubiquitylation |
Contact List
Ziqi Wang |
contact affiliation | Center for Reproductive Medicine, Shandong University, Jinan, Shandong 250012, China, |
contact email | wangziqi1110@126.com |
lab head | |
Ziqi Wang |
contact affiliation | Shandong University |
contact email | wangziqi1110@126.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD046120
- Label: PRIDE project
- Name: The deubiquitinase cofactor UAF1 interacts with USP1 and plays an essential role in spermiogenesis