PXD046107

PXD046107 is an original dataset announced via ProteomeXchange.

Title	Polyamine-dependent metabolic shielding regulates U2 snRNP SF3 subcomplex phosphorylation and alternative splicing
Description	Metabolites are central to cellular homeostasis. Whereas much emphasis has been placed on their relevance to meet energetic and biosynthetic demands, metabolic intermediates also function as signaling molecules. Here we show that polyamines, small polycations that are essential for cell biology, regulate the process of alternative splicing. We find that inhibition of polyamine synthesis increases the phosphorylation of proteins belonging to the spliceosome, consistent with a remarkable perturbation of alternative splicing in cells and tissues. Mechanistically, polyamines bind to acidic phosphorylatable motifs in splicing factors of the U2 snRNP SF3 subcomplex, thus preventing the action of kinases. In summary, we report a molecular process through which polyamines regulate protein phosphorylation in a phenomenon that we define as metabolic shielding
HostingRepository	PRIDE
AnnounceDate	2025-11-11
AnnouncementXML	Submission_2025-11-11_05:19:07.725.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Pilar Ximenez-Embun
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	phosphorylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-10-13 03:23:59	ID requested
⏵ 1	2025-11-11 05:19:08	announced

Dataset with its publication pending

submitter keyword: TMT, human phosphoproteome,poliamines, alternative splicing

Javier Muñoz
contact affiliation	Head of Proteomics Unit (affiliation at the time of MS data acquisition) Spanish National Cancer Research Centre (CNIO) Address: Melchor Fernández Almagro, 3. 28029 Madrid. SPAIN
contact email	jmunozpe@ext.cnio.es
lab head
Pilar Ximenez-Embun
contact affiliation	Proteomics Group
contact email	mpximenez@cnio.es
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD046107
     1. Label: PRIDE project
     2. Name: Polyamine-dependent metabolic shielding regulates U2 snRNP SF3 subcomplex phosphorylation and alternative splicing