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PXD046050

PXD046050 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProteome along the dorso-ventral axis of the early Drosophila embryo
DescriptionThe initially homogeneous epithelium of the early Drosophila embryo differentiates into regional subpopulations with different behaviours and physical properties that are needed for morphogenesis. The factors at top of the genetic hierarchy that control these behaviours are known, but many of their targets are not. To understand how proteins work together to mediate differential cellular activities, we studied in an unbiased manner the proteomes and phosphoproteomes of the three main cell populations along the dorso-ventral axis during gastrulation using mutant embryos that represent the different populations. We detected 6111 protein groups and 6259 phosphosites of which 3399 and 3433 respectively, were differentially regulated. The changes in phosphosite abundance did not correlate with changes in host protein abundance, showing phosphorylation to be a regulatory step during gastrulation. Hierarchical clustering of protein groups and phosphosites identified clusters that contain known fate determinants such as Doc1, Sog, Snail and Twist. The recovery of the appropriate known marker proteins in each of the different mutants we used validated the approach, but also revealed that two mutations that both interfere with the dorsal fate pathway, Toll10B and serpin27aex do this in very different manners. Diffused network analyses within each cluster point to microtubule components as one of the main groups of regulated proteins. Functional studies on the role of microtubules provide the proof of principle that microtubules have different functions in different domains along the DV axis of the embryo.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_07:00:20.480.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterHendrik Nolte
SpeciesList scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive HF-X; Q Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-10-11 00:40:04ID requested
12024-10-01 11:30:41announced
22024-10-22 07:00:20announced2024-10-22: Updated project metadata.
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Drosophila, Embryo, Development, LC-MS/MS
Contact List
Marcus Krüger
contact affiliationCECAD Research Center, University of Cologne (Institute for Genetics) Joseph-Stelzmann-Str. 26 50931 Cologne Germany
contact emailmarcus.krueger@uni-koeln.de
lab head
Hendrik Nolte
contact affiliationMax Planck Institute for Biology of Ageing, 50931 Cologne, Germany
contact emailh.nolte@age.mpg.de
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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