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PXD045938

PXD045938 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleExogenous pyruvate promotes gentamicin uptake to kill antibiotic-resistant Vibrio alginolyticus
DescriptionElucidating antibiotic resistance mechanisms is necessary for developing novel therapeutic strategies. The increasing incidence of antibiotic-resistant Vibrio alginolyticus infection threatens both human health and aquaculture, but the mechanism has not been fully elucidated. Here, an isobaric tags for relative and absolute quantification (iTRAQ) functional proteomics analysis was performed on gentamicin-resistant V. alginolyticus (VA-RGEN) and a gentamicin-sensitive strain in order to characterize the global protein expression changes upon gentamicin resistance. Proteomics analysis demonstrated a global metabolic downshift in VA-RGEN, where the pyruvate cycle (the P cycle) was severely compromised. Exogenous pyruvate restored the P cycle activity, disrupting the redox state and increasing the membrane potential. It thereby potentiated gentamicin-mediated killing by approximately 3000- and 150-fold in vitro and in vivo, respectively. More importantly, bacterial gentamicin pharmacokinetics indicated that pyruvate enhanced gentamicin influx to a degree that exceeded the gentamicin expelled by the bacteria, increasing the intracellular gentamicin. Thus, our study suggests a metabolism-based approach to combating gentamicin-resistant V. algonolyticus, which paves the way for combating other types of antibiotic-resistant bacterial pathogens.
HostingRepositoryiProX
AnnounceDate2023-09-29
AnnouncementXMLSubmission_2023-10-06_22:13:35.325.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterKuangsu Fang
SpeciesList scientific name: Vibrio alginolyticus; NCBI TaxID: 663;
ModificationListNo PTMs are included in the dataset
InstrumentTripleTOF 5600
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-10-06 22:13:23ID requested
12023-10-06 22:13:35announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Antibiotic resistance, pyruvate cycle, gentamicin, proteomics, Vibrio alginolyticus
Contact List
Bo Peng
contact affiliationSun Yat-sen University
contact emailpengb26@sysu.edu.cn
lab head
Kuangsu Fang
contact affiliationSun Yat-sen University
contact emailsufangkuang@126.com
dataset submitter
Full Dataset Link List
iProX dataset URI