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PXD045910

PXD045910 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitlePhysiological variability in mitochondrial rRNA may predispose to metabolic syndrome
DescriptionObjectives: Metabolic syndrome and its associated comorbidities are a growing concern in developed societies. Due to its polygenic nature, the genetic component of metabolic syndrome is only slowly being elucidated. Common mitochondrial DNA sequence variants have been associated with late-onset human diseases, including cardiovascular disease or type 2 diabetes mellitus, and may therefore be relevant players in the genetics of metabolic syndrome. Methods: In the present study, we investigate the effect of mitochondrial sequence variation on the metabolic phenotype in conplastic rat strains with identical nuclear but unique mitochondrial genomes that differ in the sequence of oxidative phosphorylation structural proteins, tRNAs and rRNAs. Results: Exposure to the high-fat diet led to the development of insulin resistance in the conplastic animals, which was associated with the reduced oxidative capacity of the heart (but not the liver) mitochondria. Reduced fatty acid oxidation led to the accumulation of bioactive diacylglycerols and subsequent inhibition of insulin signaling. Conclusions: We propose that these metabolic perturbations stem from the 12S rRNA sequence variation, which affects mitoribosome assembly and thus mitochondrial translation. Our work has demonstrated that physiological sequence variation in mitochondrial rRNA may be a relevant underlying factor in the progression of metabolic syndrome.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_06:54:47.386.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMarek Vrbacky
SpeciesList scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationListmethylthiolated residue
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-10-05 05:13:45ID requested
12024-08-13 04:20:07announced
22024-10-22 06:54:47announced2024-10-22: Updated project metadata.
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: metabolic syndrome
mitochondrial haplotype
diacylglycerols
insulin signaling
Contact List
Tomas Mracek
contact affiliationInstitute of Physiology of the Czech Academy of Sciences, Laboratory of Bioenergetics
contact emailtomas.mracek@fgu.cas.cz
lab head
Marek Vrbacky
contact affiliationCzech Academy of Sciences
contact emailproteom.krc@gmail.com
dataset submitter
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Dataset FTP location
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