PXD045906

PXD045906 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Cerebrospinal fluid proteome of patients with persistent pain and/or postpartum depression after elective caesarean delivery: An exploratory prospective cohort study
Description	CSF was pooled to make a study-specific QC (SPQC) sample. 500 uL of each sample was lyophilized and reconstituted in 8M urea followed by reduction/alkylation, trypsin digestion and solid phase extraction. After lyophilization, reconstituted peptides were separated by direct injection, microflowLC (1 mm ACQUITY Premier column, 100 uL/min, 60 min gradient) with post-column DMSO (3%) and interfaced to an Exploris 480 using a HESI source. MS/MS analysis used a staggered/overlapping DIA method. Additional SPQC sample was fractionated by HPRP, and 12 fractions were analyzed by microflow-DDA. A hybrid spectral library was built, and data analyzed using Spectronaut.
HostingRepository	MassIVE
AnnounceDate	2025-03-10
AnnouncementXML	Submission_2025-03-10_19:38:56.338.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Matt Foster
SpeciesList	scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606;
ModificationList	Carbamidomethyl
Instrument	Orbitrap Exploris 480

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2023-10-04 19:25:10	ID requested
⏵ 1	2025-03-10 19:38:56	announced

Publication List

no publication

no publication

Keyword List

submitter keyword: CSF, Data-independent acquisition, microflow

submitter keyword: CSF, Data-independent acquisition, microflow

Contact List

Mary Yurashevich
contact affiliation	Duke University
contact email	mary.yurashevich@duke.edu
lab head
Matt Foster
contact affiliation	Duke University
contact email	mwfoster@duke.edu
dataset submitter

Full Dataset Link List

MassIVE dataset URI
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/v06/MSV000093043/

MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/v06/MSV000093043/