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PXD045899

PXD045899 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleThe Proteomic Landscape of in vitro Cultured Endothelial Cells across Vascular Beds
DescriptionBlood vessel endothelial cells (EC) display heterogeneity across vascular beds, which is at the bases of organ specificity and anticipated to drive site-specific vascular pathology. It is assessed in vivo using transcriptomics, and in vitro using functional assays, but how proteomes compare across human in vitro cultured ECs remains incompletely characterized. We generated an in-depth human EC proteomic landscape (>8000 proteins) across 6 vascular beds and 2 in vitro models both in steady-state and upon IFNγ-induced inflammation. EC proteomes displayed a high similarity and organ specific proteins were limited. Variation between ECs and donors was mainly based in biological processes underlying proliferation and differentiation. BOECs and HUVECs represented the extremes of proteomic phenotypes. IFNγ responses were highly conserved across cellular sources. Harnessing dynamics in protein abundances we delineated interactor networks of VWF and VE-Cadherin. This EC landscape provides an extensive proteomic addition in studying EC biology and heterogeneity from an in vitro perspective.
HostingRepositoryPRIDE
AnnounceDate2024-07-25
AnnouncementXMLSubmission_2024-07-25_10:02:41.116.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterStijn Groten
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-10-04 07:24:01ID requested
12024-07-25 10:02:41announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: in vitro, cardiac, brain, aorta, lung, liver, kidney, boec, huvec,Endothelial cells
Contact List
Maartje van den Biggelaar
contact affiliationDepartment of Molecular Hematology, Sanquin Research, Amsterdam 1066CX, the Netherlands
contact emailm.vandenbiggelaar@sanquin.nl
lab head
Stijn Groten
contact affiliationSanquin Research
contact emails.groten@sanquin.nl
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
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