PXD045840 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | A framework for performing single-cell spatial metabolomics with cell-type specific protein profiling for tissue systems biology |
Description | Metabolic reprogramming in cancer and immune cells occurs to support their increasing energy needs in biological tissues. Here we propose Single Cell SPAtially resolved METabolic (scSpaMet) framework for joint protein-metabolite profiling of single immune and cancer cells in male human tissues by incorporating untargeted spatial metabolomics and targeted multiplexed protein imaging in a single pipeline. We utilized the scSpaMet pipeline to profile cell types and spatial metabolomic maps of 19507, 31156, and 8215 single cells in human lung cancer, tonsil and endometrium tissues, respectively. ScSpaMet analysis revealed cell type-dependent metabolite profiles and local metabolite competition of neighboring single cells in human tissues. Deep learning-based joint embedding revealed unique metabolite states within cell types. Trajectory inference showed metabolic patterns along cell differentiation paths. Here we show scSpaMet’s ability to quantify and visualize the cell-type specific and spatially resolved metabolic-protein mapping as an emerging tool for systems-level understanding of tissue biology. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-08 |
AnnouncementXML | Submission_2024-10-08_11:14:40.659.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | THOMAS HU |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | mass spectrometry imaging |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-10-01 10:50:35 | ID requested | |
⏵ 1 | 2024-10-08 11:14:41 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
ProteomeXchange project tag: Biology/Disease-Driven Human Proteome Project (B/D-HPP), Human Proteome Project |
submitter keyword: Human Lung, endometrium IMC imaging, tumor |
Contact List
Thomas Hu |
contact affiliation | Georgia Institute of Technology |
contact email | thu71@gatech.edu |
lab head | |
THOMAS HU |
contact affiliation | Georgia Institute of Technology |
contact email | thu71@gatech.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/10/PXD045840 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD045840
- Label: PRIDE project
- Name: A framework for performing single-cell spatial metabolomics with cell-type specific protein profiling for tissue systems biology