PXD045787

PXD045787 is an original dataset announced via ProteomeXchange.

Title	A brain-enriched circular RNA controls excitatory neurotransmission and restricts sensitivity to aversive stimuli
Description	The biological significance of circular RNAs remains largely unexplored due to the lack of loss-of-function animal models. In this study, we focused on circTulp4, a highly abundant circRNA that is enriched in the brain and synaptic compartments. We created a circTulp4-deficient (CD) mouse model and conducted a comprehensive phenotypic analysis. To test whether the electrophysiological phenotype observed in CD mice was explained by an alteration in the levels of synaptic proteins, we prepared pure synaptosomal fractions and conducted a quantitative proteomic analysis. Label-free quantification of CD and wildtype synaptic proteomes was performed by using an ion mobility-enhanced data-independent acquisition (DIA) workflow with alternating low and elevated energy (referred to as UDMSE). We quantified >1800 proteins and concluded that the protein composition of the synaptosomal compartment is preserved in CD mice.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:36:04.172.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Olaf Jahn
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2023-09-28 11:25:58	ID requested
1	2024-04-15 05:14:33	announced
⏵ 2	2024-10-22 06:36:04	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: synaptosomes, label-free proteomics, mass spectrometry, CircTulp4, phenotyping, neurophysiology,Circular RNA

Olaf Jahn
contact affiliation	Neuroproteomics Group Department of Molecular Neurobiology Max Planck Institute for Multidisciplinary Sciences Hermann-Rein-Str. 3 37075 Goettingen Germany
contact email	jahn@mpinat.mpg.de
lab head
Olaf Jahn
contact affiliation	Neuroproteomics Group Department of Molecular Neurobiology Max Planck Institute for Multidisciplinary Sciences Hermann-Rein-Str. 3 37075 Goettingen, Germany
contact email	jahn@mpinat.mpg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/04/PXD045787

PRIDE project URI

• PRIDE
  1. PXD045787
     1. Label: PRIDE project
     2. Name: A brain-enriched circular RNA controls excitatory neurotransmission and restricts sensitivity to aversive stimuli