PXD045773

PXD045773 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Generation and Characterization of a Monoclonal Antibody Against an African Swine Fever Virus Protein Encoded by the A137R Gene
Description	The ongoing African swine fever (ASF) pandemic continues to have a major impact on global pork production and trade. Since ASF cannot be distinguished from other swine hemorrhagic fevers clinically, ASF-specific laboratory diagnosis is critical. Thus ASF virus (ASFV)-specific monoclonal antibodies (mAbs) are critical for the development of laboratory diagnostics. In this study, we report one ASFV-specific mAb, F88ASF-55, that was generated and characterized. A proteomic approach was performed to determine the sequence identities of the protein band (14 kDa) recognized by F88ASF-55. The stained protein band was excised, digested, and analyzed by LC-MS/MS. A total of 10 exclusive unique peptides and 15 exclusive unique spectra were identified (data not shown). The amino acid coverage (88/137) of the structural protein encoded by A137R was 64.2%. The results confirmed that the mAb-binding epitope is located on the protein encoded by A137R (formerly known as p11.5). Epitope mapping results revealed a highly conserved linear epitope recognized by this mAb, corresponding to amino acids 111-125 of pA137R.
HostingRepository	MassIVE
AnnounceDate	2023-10-10
AnnouncementXML	Submission_2023-10-10_10:15:34.580.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Ming Yang
SpeciesList	scientific name: African swine fever virus; NCBI TaxID: 10497;
ModificationList	Carbamidomethyl
Instrument	Orbitrap Fusion Lumos

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2023-09-28 07:01:04	ID requested
⏵ 1	2023-10-10 10:15:34	announced

Publication List

no publication

no publication

Keyword List

submitter keyword: African swine fever virus, monoclonal antibody, antibody binding epitope, double-antibody sandwich ELISA, immunohistochemistry, in situ hybridization

submitter keyword: African swine fever virus, monoclonal antibody, antibody binding epitope, double-antibody sandwich ELISA, immunohistochemistry, in situ hybridization

Contact List

Ming Yang
contact affiliation	Canadian Food Inspection Agency
contact email	ming.yang@inspection.gc.ca
lab head
Ming Yang
contact affiliation	Canadian food inspection agency
contact email	mingyang@inspection.gc.ca
dataset submitter

Full Dataset Link List

MassIVE dataset URI
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MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000092995/