PXD045741 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | MmoD regulates soluble methane monooxygenase and methanobactin production in Methylosinus trichosporium OB3b |
Description | Methane oxidation by aerobic methanotrophs is well-known to be strongly regulated by the availability of copper, i.e., the “copper-switch”. That is, there are two forms of the methane monooxygenase: a cytoplasmic or soluble methane monooxygenase (sMMO) and a membrane-bound or particulate methane monooxygenase (pMMO). sMMO is only expressed and active in the absence of copper, while pMMO requires copper. Previous work has also shown that one gene in the operon of the soluble methane monooxygenase – mmoD – also plays a critical role, but its function is still vague. Herein we show that MmoD is not needed for expression of genes in the sMMO gene cluster but is critical for formation of sMMO polypeptides and sMMO activity in Methylosinus trichosporium OB3b, indicating that MmoD plays a key post-transcriptional role in maturation of sMMO. Further, data also show that MmoD controls expression of methanobactin, a unique copper-binding compound used by some methanotrophs for copper collection. Collectively these results provide greater insights into the components of the “copper-switch” and thus provide new strategies to manipulate methanotrophic activity. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_06:04:22.926.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Henriette Remmer |
SpeciesList | scientific name: Methylosinus trichosporium OB3b; NCBI TaxID: 595536; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Orbitrap Exploris 480 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-09-27 10:57:12 | ID requested | |
1 | 2023-09-29 10:07:52 | announced | |
⏵ 2 | 2024-10-22 06:04:23 | announced | 2024-10-22: Updated project metadata. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: methanotrophy, methane monooxygenase, Methylosinus trichosporium OB3b ΔmmoD, methanobactin |
Contact List
Henriette Remmer |
contact affiliation | Henriette A. Remmer, Ph.D. Director, Proteomics & Peptide Synthesis Core University of Michigan Michigan Medicine C570C MSRB II SPC 5674 |
contact email | hremmer@med.umich.edu |
lab head | |
Henriette Remmer |
contact affiliation | University of Michigan |
contact email | hremmer@umich.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD045741
- Label: PRIDE project
- Name: MmoD regulates soluble methane monooxygenase and methanobactin production in Methylosinus trichosporium OB3b