PXD045672
PXD045672 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Physiological and Cognitive Changes after Treatments of Cyclophosphamide, Methotrexate, and Fluorouracil: Implications of the Gut Microbiome and Depressive-Like Behavior |
| Description | Chemotherapy induced cognitive impairment, colloquially referred to as chemobrain, is a poorly understood phenomenon affecting a highly variable proportion of patients with breast cancer. Inflammation, a condition associated with many pathologies, is considered to be a potential mechanism behind chemobrain. Here we investigate the association between anxiety and despair-like behaviors in mice treated with cyclophosphamide, methotrexate, and fluorouracil along with host histological, proteomic, gene expression, and gut microbial responses. Forced swim and sociability tests were used to evaluate depression and despair like behaviors. Tandem mass tag proteomics approach was used to assess changes in neural protein network of the amygdala and hippocampus. While quantitative reverse transcription polymerase chain reaction, we evaluated changes in intestinal gap junction markers. Finally, the composition of gut microbiota was assessed through 16S rRNA gene sequencing. We have observed that CMF induced social and despair like behavior in mice 96 hours following treatment. Proteomic analysis identified changes various proteins related to progressive neurological disease, working memory deficit, primary anxiety disorder and gene expression revealed increases in NDMA and AMPA receptors in both the hippocampus and the amygdala as a result of CMF treatment. Finally, we observed immediate changes in the microbial population after chemotherapy treatment, with a notable increase in the mucinophilic bacteria Akkermansia mucinophila. |
| HostingRepository | MassIVE |
| AnnounceDate | 2023-09-26 |
| AnnouncementXML | Submission_2023-09-26_05:53:22.257.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Stephanie Byrum |
| SpeciesList | scientific name: Mus musculus; common name: house mouse; NCBI TaxID: 10090; |
| ModificationList | Oxidation |
| Instrument | Orbitrap Eclipse |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2023-09-25 13:59:38 | ID requested | |
| ⏵ 1 | 2023-09-26 05:53:22 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: hippocampus, chemotherapy, microbiome |
Contact List
| Antino Allen | |
|---|---|
| contact affiliation | University of Arkansas for Medical Sciences |
| contact email | arallen@uams.edu |
| lab head | |
| Stephanie Byrum | |
| contact affiliation | University of Arkansas for Medical Sciences |
| contact email | sbyrum@uams.edu |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000092957/ |
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