PXD045586

PXD045586 is an original dataset announced via ProteomeXchange.

Title	Phosphorylation of CFP10 modulates Mycobacterium tuberculosis virulence
Description	Virulence effectors secreted by Mycobacterium tuberculosis (Mtb) help subvert host immune mechanisms and, therefore, are critical for establishing infection and pathogenesis. However, knowledge in terms of signaling mechanisms that modulate the secretion of virulence factors is sparse. We performed high-throughput secretome, phosphoproteome, and phospho-secretome analysis of Mtb. We combined the analysis with empirical validations to show regulation of mycobacterial secretion through protein phosphorylation. System level PPI network analysis superimposed with the secretome, phosphoproteome, and phospho-secretome profile revealed an intricate relationship between phosphorylation and secretion. At the core of the network was a key virulence factor CFP10. We identified PknA to be the kinase responsible for phosphorylating CFP10. Using genetic tools, we show that phosphomimetic mutation of CFP10 negatively regulates the secretion of virulence mediator ESAT6. Significantly, the dynamics of CFP10 phosphorylation strongly influenced bacterial virulence and survival within macrophages and mice. Together, the results show that the dynamic phosphorylation status of the secretory protein CFP10 regulates the secretion of virulence factors and impacts virulence.
HostingRepository	PRIDE
AnnounceDate	2023-09-22
AnnouncementXML	Submission_2023-09-22_06:27:53.377.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Vinay KumarNandicoori
SpeciesList	scientific name: Mycobacterium tuberculosis H37Rv; NCBI TaxID: 83332;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-09-22 02:50:22	ID requested
⏵ 1	2023-09-22 06:27:53	announced

Dataset with its publication pending

submitter keyword: CFP10, secretion, virulence, PknA,tuberculosis, PknB, phosphorylation, ESAT6

Vinaykumar
contact affiliation	Microbial Signalling lab, CSIR-CCMB, India
contact email	vinaykn@ccmb.res.in
lab head
Vinay KumarNandicoori
contact affiliation	CCMB Director
contact email	vinaykn@ccmb.res.in
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD045586
     1. Label: PRIDE project
     2. Name: Phosphorylation of CFP10 modulates Mycobacterium tuberculosis virulence