PXD045565 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Cathepsin B Promotes Aβ Proteotoxicity by Modulating Aging Regulating Mechanisms |
Description | While the activities of certain proteases promote proteostasis and prevent neurodegeneration-associated phenotypes, the protease cathepsin B (CTSB) enhances proteotoxicity in Alzheimer’s disease (AD) model mice, and its levels are elevated in brains of AD patients. How CTSB exacerbates the toxicity of the AD-causing Amyloid β (Aβ), is controversial. Using an activity-based probe, aging-altering interventions and the nematode C. elegans we discovered that the CTSB CPR-6 promotes Aβ proteotoxicity but mitigates the toxicity of polyQ stretches. While the knockdown of cpr-6 does not affect lifespan, it alleviates Aβ toxicity by reducing the expression of swsn-3 and elevating the level of the protein SMK-1, both involved in the regulation of aging. These observations unveil a novel mechanism by which CTSB aggravates Aβ–mediated toxicity, indicate that it plays opposing roles in the face of distinct proteotoxic insults and highlight the importance of tailoring specific remedies for distinct neurodegenerative disorders. |
HostingRepository | PRIDE |
AnnounceDate | 2024-09-03 |
AnnouncementXML | Submission_2024-09-03_08:42:19.129.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Atif Ahmed |
SpeciesList | scientific name: Caenorhabditis elegans; NCBI TaxID: 6239; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-09-21 09:04:10 | ID requested | |
⏵ 1 | 2024-09-03 08:42:19 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Cathepsin activity, Neurodegeneration, C.elegans |
Contact List
Ehud Cohen |
contact affiliation | Department of Molecular Biology and Biochemistry, Institute for Medical Research Israel-Canada (IMRIC), Faculty of Medicine, Hebrew University of Jerusalem, Israel |
contact email | ehudc@ekmd.huji.ac.il |
lab head | |
Atif Ahmed |
contact affiliation | PhD student |
contact email | siddiqatif@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/09/PXD045565 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD045565
- Label: PRIDE project
- Name: Cathepsin B Promotes Aβ Proteotoxicity by Modulating Aging Regulating Mechanisms