PXD045525 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Secreted footprint of pluripotency in Man-13 HeSCs |
Description | With a view to developing a much-needed non-invasive method for monitoring the healthy pluripotent state of human stem cells in culture, we undertook proteomic analysis of the waste medium from cultured induced (Rebl.PAT) human pluripotent stem cells (hPSCs). Cells were grown in E8 medium to maintain pluripotency, and then transferred to FGF2 and TGFβ deficient E6 media for 48 hours to replicate an early, undirected dissolution of pluripotency. We identified a distinct proteomic footprint associated with early loss of pluripotency in both hPSC lines, and a strong correlation with changes in the transcriptome. We demonstrate that multiplexing of four E8- against four E6- enriched secretome biomarkers provides a robust, diagnostic metric for pluripotent state. These biomarkers were further confirmed by Western blotting which demonstrated consistent correlation with the pluripotent state across cell lines, and in response to a recovery assay. |
HostingRepository | PRIDE |
AnnounceDate | 2024-03-26 |
AnnouncementXML | Submission_2024-03-26_15:18:55.471.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Phil Lewis |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | LTQ Orbitrap Elite |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-09-20 08:33:25 | ID requested | |
⏵ 1 | 2024-03-26 15:18:55 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: pluripotency,IPSC, stem cells |
Contact List
Susan Kimber |
contact affiliation | University of Manchester |
contact email | susan.kimber@manchester.ac.uk |
lab head | |
Phil Lewis |
contact affiliation | University of Bristol |
contact email | phil.lewis@bristol.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD045525
- Label: PRIDE project
- Name: Secreted footprint of pluripotency in Man-13 HeSCs