PXD045012 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Automating data analysis for hydrogen/deuterium exchange mass spectrometry using data-independent acquisition methodology |
Description | We developed an HX-MS2 strategy that supports the acquisition of CID-generated fragment ions alongside their peptide precursors. The approach enables true auto-validation of HX data by mining the rich set of deuterated fragments, using the extra dimension of data to simultaneously confirm the peptide ID and authenticate (even correct) MS1-based deuteration calculations. The high redundancy provided by the fragments generates an opportunity to determine the confidence of deuterium calculations using a combinatorial strategy. The approach requires DIA-based acquisition methods that are available on most MS platforms, making the switch to HX-MS2 straightforward. Considerable time is saved through auto-validation and more importantly, more complex samples can now be characterized and at higher throughput, as illustrated in a drug binding analysis of the ultralarge kinase DNA-PKcs, isolated directly from mammalian cells. |
HostingRepository | PRIDE |
AnnounceDate | 2023-09-08 |
AnnouncementXML | Submission_2023-09-08_07:38:03.249.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | FrantisekFilandr |
SpeciesList | scientific name: Oryctolagus cuniculus (Rabbit); NCBI TaxID: 9986; scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Orbitrap Eclipse; Q Exactive Plus; TripleTOF 6600 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-08-31 13:55:56 | ID requested | |
⏵ 1 | 2023-09-08 07:38:03 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: DIA, LC-MS, SWATH-MS, DNA-PK,HX-MS |
Contact List
DavidC. |
contact affiliation | Department of Biochemistry & Molecular Biology, University of Calgary, Calgary, AB, Canada |
contact email | dschriem@ucalgary.ca |
lab head | |
FrantisekFilandr |
contact affiliation | University of Calgary |
contact email | frantisek.filandr@ucalgary.ca |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/09/PXD045012 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD045012
- Label: PRIDE project
- Name: Automating data analysis for hydrogen/deuterium exchange mass spectrometry using data-independent acquisition methodology