PXD044850 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Transport properties of canonical PIN proteins and the role of the loop in auxin transport |
Description | Auxin is a pivotal plant hormone that controls virtually all aspects of plant growth and development. Auxin is transported in plants in a polar fashion and PIN-FORMED (PIN) transporters are the key players in this process. Canonical PINs possess a long cytosolic loop that is subject to regulation by AGC1 and AGC3 kinases. To characterize the transporters, they are expressed in Xenopus laevis oocytes alone or with the different activating kinases. Since transport rates always depend on the amount of active transporter in the membrane it is paramount to ensure that the levels of transporter, kinase and degree of phosphorylation are similar to be able to compare transport rates directly. To this end we expressed PIN1, PIN2 and PIN3 either alone or with D6PK (an AGC1 kinase) or PID (an AGC3 kinase) in oocytes. The protein levels and the degree of phosphorylation of the loop were established and found to be comparable between PINs. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_06:56:03.336.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Julia Mergner |
SpeciesList | scientific name: Xenopus laevis (African clawed frog); NCBI TaxID: 8355; |
ModificationList | phosphorylated residue |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-08-25 02:40:28 | ID requested | |
1 | 2024-08-23 00:35:11 | announced | |
⏵ 2 | 2024-10-22 06:56:03 | announced | 2024-10-22: Updated project metadata. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Phosphoproteomics,PIN proteins, Auxin transport |
Contact List
Bernhard Kuster |
contact affiliation | Chair of Proteomics and Bioanalytics, Technical University of Munich, Germany |
contact email | kuster@tum.de |
lab head | |
Julia Mergner |
contact affiliation | Chair of Proteomics and Bioanalytics, TU Munich |
contact email | julia.mergner@tum.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD044850
- Label: PRIDE project
- Name: Transport properties of canonical PIN proteins and the role of the loop in auxin transport