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PXD044850

PXD044850 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleTransport properties of canonical PIN proteins and the role of the loop in auxin transport
DescriptionAuxin is a pivotal plant hormone that controls virtually all aspects of plant growth and development. Auxin is transported in plants in a polar fashion and PIN-FORMED (PIN) transporters are the key players in this process. Canonical PINs possess a long cytosolic loop that is subject to regulation by AGC1 and AGC3 kinases. To characterize the transporters, they are expressed in Xenopus laevis oocytes alone or with the different activating kinases. Since transport rates always depend on the amount of active transporter in the membrane it is paramount to ensure that the levels of transporter, kinase and degree of phosphorylation are similar to be able to compare transport rates directly. To this end we expressed PIN1, PIN2 and PIN3 either alone or with D6PK (an AGC1 kinase) or PID (an AGC3 kinase) in oocytes. The protein levels and the degree of phosphorylation of the loop were established and found to be comparable between PINs.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_06:56:03.336.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterJulia Mergner
SpeciesList scientific name: Xenopus laevis (African clawed frog); NCBI TaxID: 8355;
ModificationListphosphorylated residue
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-08-25 02:40:28ID requested
12024-08-23 00:35:11announced
22024-10-22 06:56:03announced2024-10-22: Updated project metadata.
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Phosphoproteomics,PIN proteins, Auxin transport
Contact List
Bernhard Kuster
contact affiliationChair of Proteomics and Bioanalytics, Technical University of Munich, Germany
contact emailkuster@tum.de
lab head
Julia Mergner
contact affiliationChair of Proteomics and Bioanalytics, TU Munich
contact emailjulia.mergner@tum.de
dataset submitter
Full Dataset Link List
Dataset FTP location
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