PXD044702

PXD044702 is an original dataset announced via ProteomeXchange.

Title	An adaptive biomolecular condensation response is conserved across environmentally divergent species
Description	Cells must sense and respond to sudden maladaptive environmental changes—stresses—to survive and thrive. Across eukaryotes, stresses such as heat shock trigger conserved responses: growth arrest, a specific transcriptional response, and biomolecular condensation of protein and mRNA into structures known as stress granules under severe stress. The composition, formation mechanism, adaptive significance, and even evolutionary conservation of these condensed structures remain enigmatic. Here we provide an unprecedented view into stress-triggered condensation, its evolutionary conservation and tuning, and its integration into other well-studied aspects of the stress response. Using three morphologically near-identical budding yeast species adapted to different thermal environments and diverged by up to 100 million years, we show that proteome-scale biomolecular condensation is tuned to species-specific thermal niches, closely tracking corresponding growth and transcriptional responses. In each species, poly(A)-binding protein—a core marker of stress granules—condenses in isolation at species-specific temperatures, with conserved molecular features and conformational changes modulating condensation. From the ecological to the molecular scale, our results reveal previously unappreciated levels of evolutionary selection in the eukaryotic stress response, while establishing a rich, tractable system for further inquiry.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:32:01.237.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	samantha keyport kik
SpeciesList	scientific name: Candida albicans (Yeast); NCBI TaxID: 5476;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2023-08-21 09:37:57	ID requested
1	2024-03-03 12:44:07	announced
⏵ 2	2024-10-22 06:32:01	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: bimolecular condensation,yeast, DIA LC-MS/MS

Sammy Keyport Kik
contact affiliation	Genetics, Genomics, and Systems Biology, The University of Chicago
contact email	skeyport@uchicago.edu
lab head
samantha keyport kik
contact affiliation	The University of Chicago
contact email	skeyport@uchicago.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD044702
     1. Label: PRIDE project
     2. Name: An adaptive biomolecular condensation response is conserved across environmentally divergent species