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PXD044313

PXD044313 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleQuantitative proteomic profiling of HCC1954 cells treated with a CDK12 degrader
DescriptionHCC1954 cells growing at 80% confluency in a 10-cm dish were treated with (R)-2-(4-(9-ethyl-6-(((6-(furan-3-yl)pyridin-3-yl)methyl)amino)-9H-purin-2-yl)morpholin-3-yl)ethan-1-ol (a CDK12 degrader) at 300 nM (0.5% in DMSO) or DMSO as a control for 2 hours. Each was carried out in triplicates. The cells were disrupted in a lysis buffer containing 2% SDS, 2 mM MgCl2, 50 mM triethylammonium bicarbonate, 1x HALT phosphatase and protease inhibitors (ThermoFisher Scientific, Waltham, MA) using Bioruptor Sonicator (Diagenode, Denville, NJ). Proteins were recovered and proteolyzed with trypsin on S-trap columns (PROTIFI, Fairport, NY). The resultant peptides were labeled with 6-plex TMT isobaric reagents (triplicates for 2 conditions), mixed altogether, subfractionated by basic reversed phase high performance liquid chromatography, and analyzed by LC-MS/MS (ThermoFisher, Q-Exactive). The LC-MS/MS data were processed by using Proteome Discoverer v2.4.
HostingRepositoryMassIVE
AnnounceDate2023-08-02
AnnouncementXMLSubmission_2023-08-02_23:38:47.928.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterNarae Park
SpeciesList scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606;
ModificationListTMT6plex; Acetyl; Oxidation; Carbamidomethyl
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-08-02 23:22:40ID requested
12023-08-02 23:38:48announced
Publication List
no publication
Keyword List
submitter keyword: LC-MS/MS based proteomics, tandem mass tag, a CDK12 degrader
Contact List
Cheolju lee
contact affiliationKorea Institute of Science and Technology
contact emailclee270@kist.re.kr
lab head
Narae Park
contact affiliationKIST
contact emailnr_park@kist.re.kr
dataset submitter
Full Dataset Link List
MassIVE dataset URI
Dataset FTP location
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