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PXD044113

PXD044113 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleThe maintenance of oocytes in the mammalian ovary involves extreme protein longevity
DescriptionIn mammals, oocytes are formed in the female embryo and need to be preserved in the ovary to ensure the viability of the next generation. How oocytes are maintained for decades is unclear. Here, we combined pulse-chase stable isotope labelling coupled with mass spectrometry, single-cell RNA-seq, microscopy and NanoSims to create an atlas of protein homeostasis in mouse oocytes and ovaries over the entire reproductive lifespan. Our results show that protein turnover in the ovary is much slower than in other organs, with hundreds of extremely long-lived proteins across a broad range of complexes and pathways, including mitochondria, ribosomes and the cytoskeleton. We propose that slow protein turnover helps to maintain proteostasis in oocytes and the ovary over long periods, protecting the germline across generations.
HostingRepositoryMassIVE
AnnounceDate2024-04-05
AnnouncementXMLSubmission_2024-04-05_12:03:12.011.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterLuisa Welp
SpeciesList scientific name: Mus musculus; common name: house mouse; NCBI TaxID: 10090;
ModificationListLabel:13C(6)
InstrumentOrbitrap Exploris 480; Orbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-07-26 10:52:09ID requested
12024-04-05 12:03:12announced
Publication List
no publication
Keyword List
submitter keyword: oocytes, long-lived proteins, SILAC
Contact List
Dr. Melina Schuh
contact affiliationMax Planck Institute for Multidisciplinary Sciences
contact emailmelina.schuh@mpinat.mpg.de
lab head
Dr. Juliane Liepe
contact affiliationMax Planck Institute for Multidisciplinary Sciences
contact emailjuliane.liepe@mpinat.mpg.de
lab head
Prof. Henning Urlaub
contact affiliationMax Planck Institute for Multidisciplinary Sciences
contact emailhenning.urlaub@mpinat.mpg.de
lab head
Luisa Welp
contact affiliationMax Planck Institute for Multidisciplinary Sciences
contact emailluisa.welp@mpinat.mpg.de
dataset submitter
Full Dataset Link List
MassIVE dataset URI
Dataset FTP location
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