PXD044113
PXD044113 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | The maintenance of oocytes in the mammalian ovary involves extreme protein longevity |
Description | In mammals, oocytes are formed in the female embryo and need to be preserved in the ovary to ensure the viability of the next generation. How oocytes are maintained for decades is unclear. Here, we combined pulse-chase stable isotope labelling coupled with mass spectrometry, single-cell RNA-seq, microscopy and NanoSims to create an atlas of protein homeostasis in mouse oocytes and ovaries over the entire reproductive lifespan. Our results show that protein turnover in the ovary is much slower than in other organs, with hundreds of extremely long-lived proteins across a broad range of complexes and pathways, including mitochondria, ribosomes and the cytoskeleton. We propose that slow protein turnover helps to maintain proteostasis in oocytes and the ovary over long periods, protecting the germline across generations. |
HostingRepository | MassIVE |
AnnounceDate | 2024-04-05 |
AnnouncementXML | Submission_2024-04-05_12:03:12.011.xml |
DigitalObjectIdentifier | |
ReviewLevel | Non peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Luisa Welp |
SpeciesList | scientific name: Mus musculus; common name: house mouse; NCBI TaxID: 10090; |
ModificationList | Label:13C(6) |
Instrument | Orbitrap Exploris 480; Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2023-07-26 10:52:09 | ID requested | |
⏵ 1 | 2024-04-05 12:03:12 | announced |
Publication List
no publication |
Keyword List
submitter keyword: oocytes, long-lived proteins, SILAC |
Contact List
Dr. Melina Schuh | |
---|---|
contact affiliation | Max Planck Institute for Multidisciplinary Sciences |
contact email | melina.schuh@mpinat.mpg.de |
lab head | |
Dr. Juliane Liepe | |
contact affiliation | Max Planck Institute for Multidisciplinary Sciences |
contact email | juliane.liepe@mpinat.mpg.de |
lab head | |
Prof. Henning Urlaub | |
contact affiliation | Max Planck Institute for Multidisciplinary Sciences |
contact email | henning.urlaub@mpinat.mpg.de |
lab head | |
Luisa Welp | |
contact affiliation | Max Planck Institute for Multidisciplinary Sciences |
contact email | luisa.welp@mpinat.mpg.de |
dataset submitter |
Full Dataset Link List
MassIVE dataset URI |
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/v06/MSV000092528/ |