PXD043911

PXD043911 is an original dataset announced via ProteomeXchange.

Title	Monitoring rapid degradation of NANOG reveals UTP15 maintains pluripotency by regulating nascent transcripts
Description	Master transcription factor (mTF) has been recognized as topmost regulatory hierarchy in cell fate determination. However, the direct transcriptional targets of mTFs, and how the interplay between these transcriptional targets and their associated RNA-binding proteins (RBPs) orchestrate the realisation of mTF functions, remain largely unknown. Here, we analysed transcriptional targets and their associated RBPs during acute depletion of NANOG in mouse embryonic stem cells (mESCs) using 5-ethynyluridine (EU) RNA metabolic labelling and click chemistry. We found that UTP15 is a sensor of NANOG transcriptional targets that maintains self-renewal of mESCs. Mechanistically, UTP15 is recruited to chromatin by NANOG transcriptional targets to maintain transcription of pluripotency-related genes. In conclusion, we provide a new paradigm to study the function of mTF and establish the mTF-nascent RNA-RBP axis that regulates cell fate decisions.
HostingRepository	PRIDE
AnnounceDate	2025-11-06
AnnouncementXML	Submission_2025-11-06_15:08:43.592.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Dongmei He
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2023-07-19 05:46:43	ID requested
1	2025-10-28 04:54:49	announced
⏵ 2	2025-11-06 15:08:44	announced	2025-11-06: Updated project metadata.

Dataset with its publication pending

submitter keyword: mouse,mESC

Xichen Bao
contact affiliation	Center for Cell Lineage and Development, CAS Key Laboratory of Regenerative Biology, Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, GIBH-HKU Guangdong-Hong Kong Stem Cell and Regenerative Medicine Research Centre, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China.
contact email	bao_xichen@gibh.ac.cn
lab head
Dongmei He
contact affiliation	University of Chinese Academy of Sciences
contact email	he_dongmei@gibh.ac.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/10/PXD043911

PRIDE project URI

• PRIDE
  1. PXD043911
     1. Label: PRIDE project
     2. Name: Monitoring rapid degradation of NANOG reveals UTP15 maintains pluripotency by regulating nascent transcripts