PXD043799 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Targeted mass spectrometry analyses of somatic mutations in colorectal cancer specimens using differential ion mobility |
Description | Identification of K-Ras and B-Raf mutations in colorectal cancer (CRC) is essential to predict patients' response to anti-EGFR therapy and formulate appropriate therapeutic strategies to improve prognosis and survival. Here, we combined parallel reaction monitoring (PRM) with high-field asymmetric waveform ion mobility (FAIMS) to enhance mass spectrometry sensitivity and improve the identification of low-abundance K-Ras and B-Raf mutations in biological samples without immunoaffinity enrichment. In targeted LC-MS/MS analyses, FAIMS reduced the occurrence of interfering ions and enhanced precursor ion purity, resulting in a three-fold improvement in the detection limit for K-Ras and B-Raf mutated peptides. In addition, ion mobility separation of isomeric peptides using FAIMS facilitated the unambiguous identification of K-Ras G12D and G13D peptides. The application of targeted LC-MS/MS analyses using FAIMS is demonstrated for the detection and quantitation of B-Raf V600E, K-Ras G12D, G13D, and G12V in CRC cell lines and primary specimens. |
HostingRepository | PRIDE |
AnnounceDate | 2023-08-14 |
AnnouncementXML | Submission_2023-08-14_04:49:52.153.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | EricBonneil |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-07-14 13:53:28 | ID requested | |
⏵ 1 | 2023-08-14 04:49:52 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: mass spectrometry, parallel reaction monitoring (PRM), BRAF,High field asymmetric waveform ion mobility (FAIMS), colorectal cancer mutations, KRAS |
Contact List
PierreThibault |
contact affiliation | IRIC-Université de Montréal |
contact email | pierre.thibault@umontreal.ca |
lab head | |
EricBonneil |
contact affiliation | Proteomic Platform |
contact email | eric.bonneil@umontreal.ca |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD043799
- Label: PRIDE project
- Name: Targeted mass spectrometry analyses of somatic mutations in colorectal cancer specimens using differential ion mobility