PXD043762

PXD043762 is an original dataset announced via ProteomeXchange.

Title	A thylakoid biogenesis BtpA protein is required for the initial step of tetrapyrrole biosynthesis in cyanobacteria
Description	Biogenesis of the photosynthetic apparatus requires complicated molecular machinery, individual components of which are either poorly characterized or unknown. The BtpA protein has been described as a factor required for the stability of Photosystem I (PSI) in cyanobacteria; however, how the BtpA stabilized PSI remains unexplained. To clarify the role of BtpA, we constructed and characterized the btpA-null mutant (btpA) in the cyanobacterium Synechocystis sp. PCC 6803. The mutant contained only ~ 1% of chlorophyll and nearly no thylakoid membranes. However, this strain, growing only in the presence of glucose, was genetically unstable and readily generate suppressor mutations that restore the photoautotrophy. Two suppressor mutations were mapped into the hemA gene encoding glutamyl-tRNA reductase (GluTR) - the first enzyme of tetrapyrrole biosynthesis. Indeed, the GluTR was not detectable in the btpA mutant and the suppressor mutations restored biosynthesis of tetrapyrroles and photoautotrophy by increased GluTR expression or by improved GluTR stability/processivity. We further demonstrated that GluTR associates with a large BtpA oligomer and that BtpA is required for the stability of GluTR. Our results show that the BtpA protein is involved in the biogenesis of photosystems on the level of regulation of tetrapyrrole biosynthesis.
HostingRepository	MassIVE
AnnounceDate	2023-10-27
AnnouncementXML	Submission_2023-10-27_06:01:01.992.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Peter Konik
SpeciesList	scientific name: Synechocystis sp. PCC 6803; NCBI TaxID: 1148;
ModificationList	Carbamidomethyl; Oxidation
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2023-07-13 07:24:32	ID requested
⏵ 1	2023-10-27 06:01:02	announced

no publication

submitter keyword: BtpA, Glutamyl-tRNA reductase, Cyanobacteria, Synechocystis sp. PCC 6803, Tetrapyrrole biosynthesis

Roman Sobotka
contact affiliation	1Laboratory of Photosynthesis, Centre Algatech, Institute of Microbiology, The Czech Academy of Sciences, 37901 Trebon; Faculty of Science, University of South Bohemia, 37005, Ceske Budejovice
contact email	sobotka@alga.cz
lab head
Peter Konik
contact affiliation	University of South Bohemia, Ceske Budejovice
contact email	konik@prf.jcu.cz
dataset submitter

MassIVE dataset URI

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