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PXD043671

PXD043671 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleNative Prohibitin Complex Enrichment
DescriptionFolding of newly synthesized proteins poses challenges for a functional proteome. Dedicated protein quality control (PQC) systems promote either the folding of nascent polypeptides at ribosomes or, if this fails, ensure their degradation. While well studied for cytosolic protein biogenesis, it is not understood how these processes work for mitochondrially-encoded proteins, key subunits of the oxidative phosphorylation system (OXPHOS). Here, we identify dedicated hubs in proximity to mitoribosomal tunnel exits coordinating mitochondrial translation, protein import, assembly, and protein turnover. Conserved prohibitin/m-AAA protease supercomplexes and the availability of assembly chaperones determine the fate of newly synthesized proteins by molecular triaging. The localization of these competing activities in vicinity to the mitoribosomal tunnel exit allows for a prompt decision whether newly synthesized proteins are fed into OXPHOS assembly or degraded. This repository contains the results of the FLAG-based enrichment of the native prohibitin complex. Please note that we measured elution either with LDS sample buffer or using FLAG-peptides. In the published study, the LDS elution buffer was shown.
HostingRepositoryPRIDE
AnnounceDate2023-08-14
AnnouncementXMLSubmission_2023-08-14_00:42:59.335.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterHendrikNolte
SpeciesList scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationListNo PTMs are included in the dataset
InstrumentOrbitrap Exploris 480
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-07-11 05:03:28ID requested
12023-08-14 00:42:59announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Yeast, Immunoprecipitation,Prohibitin, Native complex
Contact List
ThomasLanger
contact affiliationMax-Planck-Institute for Biology of Ageing Department of Mitochondrial Proteostasis Joseph-Stelzmann-Str. 9b 50931 Cologne
contact emailtlanger@age.mpg.de
lab head
HendrikNolte
contact affiliationMax Planck Institute for Biology of Ageing, 50931 Cologne, Germany
contact emailh.nolte@age.mpg.de
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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