PXD043639

PXD043639 is an original dataset announced via ProteomeXchange.

Title	Mitochondrial RelA empowers mtDNA G-quadruplex formation for hypoxia adaptation in cancer cells
Description	Mitochondrial DNA (mtDNA) G-quadruplexes (G4s) have been proposed as potential regulators of energy metabolism. However, numerous aspects of this process remain elusive. In our quest, employing a chemical-genetic screening strategy, we successfully identified the JAK/STAT3 pathway as the primary regulatory mechanism controlling mtDNA G4 dynamics in hypoxic MCF-7 cells. Further proteomic analyses and associated investigations have shed light on the translocation of RelA, a member of the NF-κB family, to the mitochondria upon activation of the JAK/STAT3 pathway. Once localized, RelA directly binds to mtDNA G4s and facilitates their folding, consequently yielding escalated mtDNA instability, impaired mtDNA transcription, and ensuing mitochondrial dysfunction. Ultimately, this intricately orchestrated cascade disrupts the equilibrium of energy metabolism, favoring a more pronounced deviation towards glycolysis. This pioneering revelation introduces an unprecedented and pivotal component to the intricately interconnected framework harnessed by cancer cells, empowering them to undergo metabolic reprogramming and adapt to hypoxic environments.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:38:58.217.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tang Guixue
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2023-07-10 06:05:29	ID requested
1	2024-05-13 01:05:57	announced
⏵ 2	2024-10-22 06:38:58	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: huamn, G-quadruplex, mitochondria, LC-MS/MS

Tan Jiaheng
contact affiliation	School of Pharmaceutical Science, Sun Yat-Sen University, China
contact email	tanjiah@mail.sysu.edu.cn
lab head
Tang Guixue
contact affiliation	Sun Yat-Sen University
contact email	tanggx6@mail2.sysu.edu.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/05/PXD043639

PRIDE project URI

• PRIDE
  1. PXD043639
     1. Label: PRIDE project
     2. Name: Mitochondrial RelA empowers mtDNA G-quadruplex formation for hypoxia adaptation in cancer cells