PXD043574
PXD043574 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Multi-omics analysis using antibody-based in situ biotinylation technique reveals the mechanism of Cajal body formation |
| Description | Membrane-less subcellular compartments such as cytoplasmic bodies, nuclear bodies, and paraspeckles play important roles in a variety of cellular functions. Although many of the techniques for identifying the components of cellular bodies were developed to address their function, no technique to comprehensively identify the components of these bodies in both static and dynamic states has been established. Here, we developed an antibody-based in situ biotinylation proximity-labeling technique and succeeded in identifying the components of both static and dynamic nuclear bodies, nucleolus and γH2AX foci, respectively. Using this technique, we comprehensively identified the components of Cajal bodies (CBs), including DNAs, RNAs, and proteins, and clarified their interactome in CBs. Furthermore, we captured the dynamic change of CBs by inhibiting transcription. Our analysis revealed that nascent snRNAs transcribed in CBs play a role in CB nucleation through assembling RNA binding proteins, including frontotemporal dementia-related proteins, RBMs and HNRNPs. |
| HostingRepository | jPOST |
| AnnounceDate | 2024-07-06 |
| AnnouncementXML | Submission_2024-07-05_08:00:04.735.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Hidehisa Takahashi |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
| ModificationList | L-methionine sulfoxide; S-carboxamidomethyl-L-cysteine; alpha-amino acetylated residue; O-phospho-L-serine; O-phospho-L-threonine; O4'-phospho-L-tyrosine |
| Instrument | Q Exactive; LTQ Orbitrap Elite |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2023-07-05 23:12:37 | ID requested | |
| ⏵ 1 | 2024-07-05 08:00:05 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: Cajal body, nucleolus, γH2AX, small nuclear RNA, biotinylation |
Contact List
| Hidehisa Takahashi | |
|---|---|
| lab head | |
| Hidehisa Takahashi | |
| contact affiliation | Yokohama city university |
| dataset submitter | |
Full Dataset Link List
| jPOST dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST002223/ |
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