PXD043539

PXD043539 is an original dataset announced via ProteomeXchange.

Title	Phosphoproteome analysis of TgPP1 depleted Toxoplasma gondii tachyzoites
Description	Virulence of apicomplexan parasites is based on their ability to divide rapidly to produce significant biomass. The regulation of their cell-cycle is therefore key to their pathogenesis. Phosphorylation is a crucial post-transcriptional modification that regulates many aspects of the cell cycle. The phosphatase PP1 is known to play a major role in the phosphorylation balance in eukaryotes. We explored the role of TgPP1 during the cell cycle of the tachyzoite form of the apicomplexan parasite Toxoplasma gondii. Using a conditional mutant strain, we show that TgPP1 regulates many aspects of the cell cycle including the production of the daughter cells inner membrane complex (IMC), the segregation of organelles and the nuclear division. Unexpectedly, depletion of TgPP1 also results in the accumulation of amylopectin, a storage polysaccharide that is normally found in the latent bradyzoite form of the parasite. Using transcriptomics and phosphoproteomics, we show that TgPP1 mainly acts through post-transcriptional mechanisms by dephosphorylating target proteins including IMC proteins. TgPP1 also dephosphorylate a protein bearing a starch binding domain. Mutagenesis analysis reveals that the targeted phosphor-sites are linked to the ability of the parasite to produce amylopectin in conditions inducing bradyzoite differentiation. Therefore, we show that TgPP1 have pleiotropic roles during the tachyzoite cell cycle regulation, but also regulates amylopectin accumulation.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:53:25.427.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Cerina Chhuon
SpeciesList	scientific name: Toxoplasma gondii GT1; NCBI TaxID: 507601; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2023-07-04 10:25:21	ID requested
1	2024-08-08 11:04:46	announced
⏵ 2	2024-10-22 06:53:25	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: phosphorylation,Phosphoproteome, toxoplasma, parasites, LC-MS/MS

Ida Chiara Guerrera
contact affiliation	Proteomics platform Necker, Université Paris Cité - Structure Fédérative de Recherche Necker, INSERM US24/CNRS UAR3633, Paris 75015, France.
contact email	chiara.guerrera@inserm.fr
lab head
Cerina Chhuon
contact affiliation	Proteomics Platform Necker, PPN-3P5, Structure Fédérative de Recherche SFR Necker, Université Paris Descartes, Paris, France
contact email	cerina.chhuon@inserm.fr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD043539
     1. Label: PRIDE project
     2. Name: Phosphoproteome analysis of TgPP1 depleted Toxoplasma gondii tachyzoites