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PXD043317

PXD043317 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleSyphilis and the Host; multi-omic analysis of host cellular responses to Treponema pallidum provides novel insight into syphilis pathogenesis.
DescriptionWhole cell lysates from hCMEC/d3 brain microvascular endothelial cells grown in medium and heavy SILAC media and exposed to Treponema pallidum or control media for 5 hours. Prior to Mass-spec analysis, each sample was fractionated into 12 fractions via high-pH reversed-phase liquid chromatography, then each fraction was analyzed by liquid chromatography tandem mass spectrometry. Exposures were repeated with 4 biological replicates, which we define as individual sample wells. In samples 1&2 medium SILAC cells were exposed to Treponema pallidum, and heavy SILAC cells were exposed to control media. In Samples 3&4 medium SILAC cells were exposed to control media, and heavy SILAC cells were exposed to Treponema pallidum. Cells were grown in D4-lysine (37.5 mg/liter) and 13C6-arginine (21.75 mg/liter) for medium isotope-labeled cells, or 13C615N2-lysine (38.5 mg/liter) and 13C615N4-arginine (22.25 mg/liter) for heavy isotope-labeled cells.
HostingRepositoryMassIVE
AnnounceDate2023-09-13
AnnouncementXMLSubmission_2023-09-13_14:58:10.395.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterSean
SpeciesList scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606;
ModificationListunknown modification
InstrumentOrbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-06-26 16:37:33ID requested
12023-09-13 14:58:10announced
Publication List
Waugh S, Ranasinghe A, Gomez A, Houston S, Lithgow KV, Eshghi A, Fleetwood J, Conway K, Reynolds LA and Cameron CE. Syphilis and the host
multi-omic analysis of host cellular responses to Treponema pallidum provides novel insight into syphilis pathogenesis. Front. Microbiol. 14:1254342. doi: 10.3389/fmicb.2023.1254342.
Keyword List
submitter keyword: syphilis, endothelial, Treponema pallidum
Contact List
Dr. Caroline Cameron
contact affiliationUniversity of Victoria
contact emailcaroc@uvic.ca
lab head
Sean
contact affiliationUniversity of victoria
contact emailswaugh@uvic.ca
dataset submitter
Full Dataset Link List
MassIVE dataset URI
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000092275/