PXD043260

PXD043260 is an original dataset announced via ProteomeXchange.

Title	A conserved membrane curvature-generating protein is crucial for autophagosome formation in fission yeast
Description	Organelles are shaped by curvature-generating proteins, which include the reticulons and REEPs that are involved in forming the endoplasmic reticulum (ER). A conserved REEP subfamily differs from the ER-shaping REEPs in abundance and membrane topology and has unidentified functions. Here, we show that Rop1, the single member of this family in the fission yeast Schizosacharomyces pombe, is crucial for the macroautophagy of organelles and cytosolic proteins. Rop1 is needed for the formation of phagophores, cup-like structures consisting of two closely apposed membrane sheets that encapsulate cargo. It is recruited at early stages to phagophores and is required for their maturation into autophagosomes. Rop1 function relies on its ability to generate high membrane curvature and on its colocalization with the autophagy component Atg2 that is thought to reside at the phagophore rim. We propose that Rop1 facilitates the formation and growth of the double-membrane structure of the autophagosome.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:59:48.963.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Joao Paulo
SpeciesList	scientific name: Schizosaccharomyces pombe 927; NCBI TaxID: 1264690;
ModificationList	monohydroxylated residue
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2023-06-24 05:31:32	ID requested
1	2023-08-06 13:14:08	announced
⏵ 2	2024-10-22 05:59:49	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: REEP1, autophagy, fission yeast

Tom Rapoport
contact affiliation	Harvard Medical School Department of Cell Biology Boston MA 02115, USA
contact email	tom_rapoport@hms.harvard.edu
lab head
Joao Paulo
contact affiliation	Harvard Medical School
contact email	joao_paulo@post.harvard.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD043260
     1. Label: PRIDE project
     2. Name: A conserved membrane curvature-generating protein is crucial for autophagosome formation in fission yeast